{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:06:00Z","timestamp":1774915560918,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T00:00:00Z","timestamp":1566172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Astragaloside IV (AS\u2010IV), a natural herbal compound from<jats:italic>Astragalus membranaceus<\/jats:italic>, has inhibitory effects on receptor activator of nuclear factor\u2010\u03baB ligand (RANKL)\u2010induced osteoclastogenesis, and RANKL signal helps to regulate odontoblast differentiation. However, whether and how AS\u2010IV affects odontoblastic differentiation remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lipopolysaccharide (LPS)\u2010stimulated MDPC\u201023 cells and rat pulpitis were treated with AS\u2010IV, cell viability, and LDH leakage was analyzed by CCK\u20108 assay and LDH Leakage assay. The production of TNF\u2010\u03b1 and IL\u20106 was determined by ELISA and qRT\u2010PCR assay. The expression of alkaline phosphatase (ALP) was detected using an ALP assay kit, and the expression of dentin sialophos\u2010phoprotein (DSPP), dentin matrix protein\u20101 (DMP1), basic fibroblast growth factor (FGF2), and phosphorylated extracellular signal\u2010regulated kinase (p\u2010ERK) was determined by western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>AS\u2010IV dose dependently increased in cell viability and decreased the overproduction of TNF\u2010\u03b1 and IL\u20106 in LPS\u2010stimulated MDPC\u201023 cells. AS\u2010IV also counteracted LPS\u2010induced downregulation of ALP, DSPP, and DMP1 in MDPC\u201023 cells. Furthermore, AS\u2010IV significantly decreased the expression of FGF2 and p\u2010ERK in LPS\u2010stimulated MDPC\u201023 cells. More important, the addition of FGF2 partly neutralized AS\u2010IV\u2010mediated inhibition of FGF2\/ERK signaling, abolished AS\u2010IV\u2010induced reduction of TNF\u2010\u03b1 and IL\u20106, and counteracted AS\u2010IV\u2010induced upregulation of DSPP and DMP\u20101 in these cells. Meanwhile, AS\u2010IV inhibited the excessive production of TNF\u2010\u03b1 and IL\u20106, suppressed the downregulation of DSPP and DMP1, and disturbed the up\u2010regulation of FGF2 and p\u2010ERK in the pulp tissues of rat pulpitis model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>AS\u2010IV exerted anti\u2010inflammatory and pro\u2010differentiation effects in LPS\u2010stimulated MDPC\u201023 cells and rat pulpitis via inhibiting the FGF2\/ERK signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12926","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T18:24:21Z","timestamp":1563474261000},"page":"951-958","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Astragaloside IV attenuates inflammatory injury and promotes odontoblastic differentiation in lipopolysaccharide\u2010stimulated MDPC\u201023 cells and rat 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