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These structures are produced by many different cell types and can modulate cellular activity by induction of epigenetic alterations. These vesicles may promote tumor mass increase either by stimulating cell proliferation <jats:italic>via<\/jats:italic> growth factors or by inhibiting apoptosis, which reinforces the role of such vesicles as important modulators of tumor progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The present in vitro study aimed to characterize MV derived from malignant neoplastic epithelial cell cultures (EP) and their effect on the expression of apoptosis\/autophagy and invasion related genes of benign myoepithelial (Myo) cell cultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results revealed round structures with a mean size of 153.6 (\u00b10.2) nm, with typical MV morphology. CD63 quantification indicated that EP cell culture at 70%\u201080% confluence secreted 3.088\u00a0\u00d7\u00a010<jats:sup>8<\/jats:sup> MV\/mL. Overall, Myo exposed to MVs derived from EP showed both up\u2010 and downregulation of tumorigenesis promoting genes. MVs from EP cells promoted cell death of Myo cells and positively modulate <jats:italic>BAX<\/jats:italic>, <jats:italic>SURVIVIN<\/jats:italic>, <jats:italic>LC3B, MMP\u20102,<\/jats:italic> and <jats:italic>MMP\u20109<\/jats:italic> expression. Furthermore, an increasing of MMP\u20102 and MMP\u20109 secretion by Myo was observed after MV exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that MVs from EP modulate autophagy of Myo cells, which may, in part, explain the disappearance of these cells in in situ areas of invasive carcinoma ex\u2010pleomorphic adenoma. Additionally, the overexpression of MMPs contributes to the development of an invasive phenotype of Myo cells, which could favor the dissolution of the basement membrane during tumorigenesis process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13037","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T19:56:06Z","timestamp":1590522966000},"page":"761-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Microvesicles derived from squamous cell carcinoma induce cell death, autophagy, and invasion of benign myoepithelial cells"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4991-1185","authenticated-orcid":false,"given":"Elizabeth Ferreira","family":"Martinez","sequence":"first","affiliation":[{"name":"Cell Biology and Oral Pathology Division Faculdade S\u00e3o Leopoldo Mandic Campinas SP 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