{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T08:21:00Z","timestamp":1771575660733,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,6,23]],"date-time":"2020-06-23T00:00:00Z","timestamp":1592870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mucous membrane pemphigoid (MMP) is an uncommon bullous disease typically involving the oral cavity. The most commonly used laboratory test for the diagnosis of MMP is direct immunofluorescence (DIF) on fresh perilesional tissue; however, the sensitivity of this test may be hampered by technical difficulties. Immune\u2010serological investigations can also be employed to render a diagnosis. The purpose of this paper was to present an evaluation of diagnostic testing for MMP within an Oral Medicine Unit in UK.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A retrospective analysis of the medical records was undertaken for patients who had undergone biopsy and DIF testing from January 2016 to December 2018. Parameters analysed included clinical presentation, histopathological features, DIF, salt\u2010split skin indirect immunofluorescence, ELISA anti\u2010BP180 and BP 230 and HLA\u2010DQB1*03:01 findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirty patients (23 females and 7 males, mean age 66.8\u00a0years old) were diagnosed with MMP through a combination of histopathology and serological testing. Sixteen patients (53%) were DIF positive, whereas in 14 (47%), MMP diagnosis was achieved using immune\u2010serologic tests. HLA DQB1*03:01 status was undertaken in 15 DIF\u2010positive and 12 DIF\u2010negative patients, and HLA DQB1*03:01 was found in 73% and 58% of the cases, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This service evaluation has shown that when DIF is informative, it remains the gold standard technique for diagnosis of MMP. However, we have also highlighted the value of serological testing for increasing diagnostic yield for patients with suspected MMP and the potential for HLA DQB1*03:01 as an adjunctive test for the evaluation of MMP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13054","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T21:10:47Z","timestamp":1591737047000},"page":"687-692","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A service evaluation of the diagnostic testing for mucous membrane pemphigoid in a UK oral medicine unit"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1759-7483","authenticated-orcid":false,"given":"Richard","family":"Walton","sequence":"first","affiliation":[{"name":"Department of Oral Medicine Newcastle School of Dental Sciences Newcastle University Newcastle UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology Royal Victoria Infirmary Newcastle UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Carrozzo","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Newcastle School of Dental Sciences Newcastle University Newcastle UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61140-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.138.3.370"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12193"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.10.007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.16.7747"},{"issue":"5","key":"e_1_2_8_7_1","first-page":"330","article-title":"HLA\u2010DRB1 and HLA\u2010DQB1 genes in susceptibility and resistance to cicatricial pemphigoid in French Caucasians","volume":"8","author":"Drouet M","year":"1998","journal-title":"Eur J Dermatol"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2001.04380.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04448.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41433-018-0092-5"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1986.tb04544.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2050.2004.00398.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ico.0000178730.34564.c7"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0736-8046.2004.21110.x"},{"issue":"5","key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"S128","DOI":"10.1067\/mjd.2002.107967","article-title":"Cicatricial pemphigoid\u2010induced phimosis","volume":"46","author":"Fueston JC","year":"2001","journal-title":"J Am Acad Dermatol"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1988.tb01744.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1988.tb01776.x"},{"issue":"2","key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1111\/ddg.12716","article-title":"Diagnostic relevance of direct immunofluorescence in ocular mucous membrane pemphigoid","volume":"13","author":"Mehra T","year":"2015","journal-title":"J Dtsch Dermatol Ges"},{"issue":"5","key":"e_1_2_8_19_1","first-page":"E287","article-title":"Sensitivity of direct immunofluorescence in oral diseases. 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