{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T19:47:48Z","timestamp":1778010468772,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,18]],"date-time":"2020-07-18T00:00:00Z","timestamp":1595030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Odontogenic keratocyst (OKC) is a unique developmental odontogenic cyst that has the potential to behave aggressively and is associated with the nevoid basal cell carcinoma syndrome. Orthokeratinized odontogenic cyst (OOC) is a distinct, uncommon odontogenic cyst. It significantly differs from OKC not only in its epithelial lining but also in proliferating kinetics, clinical, immunohistochemical and biological behaviour. <jats:italic>BRAF<\/jats:italic> gene located on chromosome 7q34 encodes a cytoplasmic serine\u2010threonine kinase. Various immunohistochemical studies have been conducted to express the <jats:italic>BRAFV600E<\/jats:italic> gene mutation in various odontogenic cyst and tumours with varying results. The present study was conducted to evaluate the possible role of <jats:italic>BRAFV600E<\/jats:italic> in the pathogenesis of sporadic OKC, syndromic OKC and OOC by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue blocks of 15 diagnosed cases each of sporadic OKC, syndromic OKC and OOC were retrieved from the archives of Department of Oral Pathology and subjected to immunohistochemical staining for the detection of <jats:italic>BRAFV600E<\/jats:italic> mutation using a novel rabbit monoclonal antibody clone RM8.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunohistochemical analysis showed complete absence of <jats:italic>BRAFV600E<\/jats:italic> mutation in all cases of sporadic OKC, syndromic OKC and OOC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The negative immunohistochemical expression of <jats:italic>BRAFV600E<\/jats:italic> in sporadic OKC, syndromic OKC and OOC suggests that <jats:italic>BRAFV600E<\/jats:italic> plays no role in the pathogenesis of sporadic OKC, syndromic OKC and OOC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13081","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T09:40:28Z","timestamp":1593250828000},"page":"1061-1067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Absence of <i>BRAFV600E<\/i> immunohistochemical expression in sporadic odontogenic keratocyst, syndromic odontogenic keratocyst and orthokeratinized odontogenic cyst"],"prefix":"10.1111","volume":"49","author":[{"given":"Kejal S.","family":"Jain","sequence":"first","affiliation":[{"name":"Department of Oral Pathology and Microbiology Nair Hospital Dental College Mumbai 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