{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:10Z","timestamp":1772160730989,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The current diagnostic standard for detection of high\u2010risk human papillomavirus (HPV) in oropharyngeal squamous cell carcinoma is via a two\u2010stage algorithm, namely p16 immunohistochemistry followed by HPV DNA in situ hybridization in p16 positive cases. This study evaluated the feasibility of automated RNA in situ hybridization on a clinical platform as a single\u2010step alternative to the two\u2010stage algorithm within a routine diagnostic histopathology setting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Thirty\u2010eight cases positive for both p16 and DNA in situ hybridization, 42 p16 negative cases and 20 cases positive for p16 but negative for DNA in situ hybridization were randomly selected. High\u2010risk HPV RNA in situ hybridization was undertaken on all cases on an automated clinical platform. Manufacturer\u2010recommended and on\u2010slide additional p16\/HPV positive and negative controls were used. Test quality assurance and diagnostic RNA in situ hybridization were independently assessed by two observers. A consensus diagnosis was reached in the presence of a third observer on discordant cases. All RNA in situ hybridization results were then correlated against p16 and DNA ISH status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Inter\u2010slide RNA in situ hybridization staining variation was observed in control sections. RNA in situ hybridization demonstrated a high inter\u2010observer agreement rate (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u00a0.897,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001). Following consensus review, there was full concordance between RNA in situ hybridization and the current standard.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Human papillomavirus testing by standalone automated RNA in situ hybridization on a clinical diagnostic platform currently available in routine diagnostic histopathology laboratories is a feasible alternative to the two\u2010step algorithm of p16 and DNA in situ hybridization. Control tissue staining procedures need to be adapted to achieve the most accurate results.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13103","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T15:06:38Z","timestamp":1598367998000},"page":"68-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["RNA in situ hybridization for human papillomavirus testing in oropharyngeal squamous cell carcinoma on a routine clinical diagnostic platform"],"prefix":"10.1111","volume":"50","author":[{"given":"Rhonda","family":"Henley\u2010Smith","sequence":"first","affiliation":[{"name":"Head and Neck Cancer Biobank Guy's and St Thomas' NHS Foundation Trust London UK"},{"name":"Head and Neck Pathology Guy's and St Thomas' NHS Foundation Trust London UK"},{"name":"Centre for Host Microbiome Interactions Faculty of Dental, Oral and Craniofacial Science King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Santambrogio","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology Faculty of Dentistry, Oral &amp; Craniofacial Sciences King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia L.","family":"Andoniadou","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology Faculty of Dentistry, Oral &amp; Craniofacial Sciences King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Odell","sequence":"additional","affiliation":[{"name":"Head and Neck Pathology Guy's and St Thomas' NHS Foundation Trust London UK"},{"name":"Centre for Host Microbiome Interactions Faculty of Dental, Oral and Craniofacial Science King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5720-7422","authenticated-orcid":false,"given":"Selvam","family":"Thavaraj","sequence":"additional","affiliation":[{"name":"Head and Neck Pathology Guy's and St Thomas' NHS Foundation Trust London UK"},{"name":"Centre for Clinical, Oral and Translational Science Faculty of Dental, Oral and Craniofacial Science King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdx304"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-017-9686-9"},{"key":"e_1_2_10_4_1","volume-title":"WHO classification of head and neck tumours","author":"El\u2010Naggar AK","year":"2017"},{"key":"e_1_2_10_5_1","volume-title":"TNM classification of malignant tumours","author":"Brierley J","year":"2017"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-40618-3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2020.104652"},{"key":"e_1_2_10_8_1","unstructured":"National Institute for Health and Care Excellence (NICE).Cancer of the upper aerodigestive tract: assessment and management in people aged 16 and over.2016.https:\/\/www.nice.org.uk\/guidance\/ng36\/chapter\/Recommendations#hpvrelated\u2010disease. 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