{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:12Z","timestamp":1772160732874,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T00:00:00Z","timestamp":1604016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>CKS2<\/jats:italic>\n                      (CDC28 Protein Kinase Regulatory Subunit 2) is a gene that encodes CKS2 protein that has been characterized as a binding partner of the\u00a0catalytic\u00a0subunit\u00a0of\u00a0the\u00a0cyclin\u2010dependent\u00a0kinases. However, its expression profile and regulatory effects in tongue squamous cell carcinoma has not yet been explored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bioinformatic analysis was conducted using bulk\u2010seq data from The Cancer Genome Atlas and single\u2010cell RNA\u2010seq data from GSE103322. SCC9 and CAL27 cells were used as in vitro cell models for cellular and molecular studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>CKS2<\/jats:italic>\n                      expression was significantly upregulated in tongue squamous cell carcinoma tissues (N\u00a0=\u00a0128) compared with adjacent normal tissues (N\u00a0=\u00a013). Its upregulation was associated with significantly shorter disease\u2010specific survival and progression\u2010free survival. Cellular status estimation in tumor cells indicated that\n                      <jats:italic>CKS2<\/jats:italic>\n                      expression was moderately and positively correlated with cell\u2010cycle progression.\n                      <jats:italic>CKS2<\/jats:italic>\n                      inhibition in SCC9 and CAL27 cells resulted in decreased proliferation, weakened colony formation capability, and cell\u2010cycle arrest at the G2\/M phase. Immunofluorescence staining and co\u2010Immunoprecipitation (co\u2010IP) assay confirmed co\u2010localization and interaction between CKS2 and DUTPase.\n                      <jats:italic>CKS2<\/jats:italic>\n                      knockdown did not alter DUTPase expression but reduced its nuclear distribution. Both\n                      <jats:italic>CKS2<\/jats:italic>\n                      and\n                      <jats:italic>DUT<\/jats:italic>\n                      expression were moderately correlated with their gene\u2010level copy number.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>CKS2<\/jats:italic>\n                      expression is associated with unfavorable survival of patients with tongue squamous cell carcinoma. Inhibiting its expression could reduce tongue squamous cell carcinoma cell growth and induce G2\/M arrest. CKS2 may interact with DUTPase and regulate its nuclear localization. Gene\u2010level copy amplification might be an important mechanism of upregulated\n                      <jats:italic>CKS2<\/jats:italic>\n                      and\n                      <jats:italic>DUT<\/jats:italic>\n                      in the tumor.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13116","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T11:02:19Z","timestamp":1603796539000},"page":"175-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["CKS2 modulates cell\u2010cycle progression of tongue squamous cell carcinoma cells partly via modulating the cellular distribution of DUTPase"],"prefix":"10.1111","volume":"50","author":[{"given":"Fei","family":"Gao","sequence":"first","affiliation":[{"name":"Operation Room Jinan Stomatological Hospital Jinan 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