{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:12:03Z","timestamp":1781845923670,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T00:00:00Z","timestamp":1610928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>BRAF<\/jats:italic>\n                      p.V600E is reported in up to 80% of ameloblastomas. Despite the high frequency, the presence of this mutation in different histopathological areas of the tumour has not been investigated. This information has an important role in the use of\n                      <jats:italic>BRAF<\/jats:italic>\n                      p.V600E assessment as an auxiliary tool in the differential diagnosis between unicystic ameloblastoma and other odontogenic cystic lesions, especially when only incisional biopsies are available. Therefore, the purpose of the present study was to investigate\n                      <jats:italic>BRAF<\/jats:italic>\n                      p.V600E heterogeneity in unicystic ameloblastoma.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Five cases of ameloblastoma and two dentigerous cysts were analysed. The regions exhibiting different microscopic characteristics were selected from each ameloblastoma case and manually dissected. TaqMan allele\u2010specific qPCR or Sanger sequencing was performed to determine\n                      <jats:italic>BRAF<\/jats:italic>\n                      p.V600E status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We screened the mutation in a small cohort of UA and no molecular heterogeneity was found. Four cases of ameloblastoma (80%) exhibited\n                      <jats:italic>BRAF<\/jats:italic>\n                      p.V600E in all different areas evaluated. One case did not harbour the mutation in any microscopic region analysed. The\n                      <jats:italic>BRAF<\/jats:italic>\n                      mutation was absent in the dentigerous cysts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Ameloblastomas appear to exhibit a homogeneous profile regarding the\n                      <jats:italic>BRAF<\/jats:italic>\n                      p.V600E no matter what histological feature is observed under light microscopy, suggesting that this molecular test may contribute to establish the correct diagnosis in cases microscopically resembling other odontogenic lesions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13155","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T16:46:33Z","timestamp":1609346793000},"page":"478-484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<i>BRAF<\/i>\n                    p.V600E status in epithelial areas of ameloblastoma with different histological aspects: Implications to the clinical practice"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-1884","authenticated-orcid":false,"given":"Maria Sissa Pereira","family":"Sant\u2019Ana","sequence":"first","affiliation":[{"name":"Department of Oral Surgery and Pathology School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Ferreira","family":"dos Santos Costa","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and 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