{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T17:15:22Z","timestamp":1784135722590,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of miR\u2010626 in oral squamous cell carcinoma (OSCC) was investigated by targeting RASSF4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The miR\u2010626 and RASSF4 expression was detected in normal oral mucosa or OSCC tissues and OSCC or normal cells. The methylation status of RASSF4 was analyzed using methylation\u2010specific polymerase chain reaction (PCR). The cytoplasmic\/nuclear ratios (C\/N ratios) targeted by miR\u2010626 were examined using microarray, followed by a dual\u2010luciferase reporter assay. The subcellular localization of RASSF4 and miR\u2010626 in OSCC cells was determined using RNA fluorescence in situ hybridization (FISH) and immunocytochemistry (ICC), respectively. Ca9\u201022 and HSC2 cells were divided into mock, inhibitor NC, miR\u2010626 inhibitor, scramble, RASSF4 and miR\u2010626 mimic\u00a0+\u00a0RASSF4\u00a0groups, and then CCK\u20108, Annexin V\u2010FITC\/PI, wound healing, Transwell, qRT\u2010PCR and western blotting assays were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>OSCC tissues and cells had increased miR\u2010626 expression and decreased RASSF4 expression. Patients with RASSF4 methylation had lower RASSF4 expression than those without methylation. In addition, a negative correlation between miR\u2010626 and RASSF4 was found in OSCC tissues, both of which were correlated with the pathological grade, pathological stage, lymph node metastasis and patient prognosis. MiR\u2010626 targeted RASSF4 in OSCC cells. Overexpressed RASSF4 inhibited the proliferation, invasion, migration and epithelial\u2013mesenchymal transition (EMT) of OSCC cells, promoted cell apoptosis, and blocked the Wnt\/\u03b2\u2010Catenin pathway, which was reversed by miR\u2010626 overexpression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Inhibiting miR\u2010626 can regulate the biological characteristics of OSCC cells, including proliferation, invasion, migration, EMT and apoptosis, by targeting RASSF4, which may be related to the Wnt\/\u03b2\u2010Catenin pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13216","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T08:24:37Z","timestamp":1623659077000},"page":"1005-1017","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Wnt\/\u03b2\u2010catenin signaling pathway participates in the effect of miR\u2010626 on oral squamous cell carcinoma 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