{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:59:27Z","timestamp":1758124767811,"version":"3.40.5"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,10,3]],"date-time":"2021-10-03T00:00:00Z","timestamp":1633219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81702708"],"award-info":[{"award-number":["81702708"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although transfer RNA (tRNA) has been found to be the main source of a rich class of noncoding RNA, the tRNA\u2010derived small RNA (tsRNA) has been proved to play an irreplaceable role in the human body, and its dynamic imbalance could affect the progress of the disease. However, the research on tsRNA in oral submucous fibrosis (OSF) is still scarce.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We sequenced the OSF and validated it by PCR. We found that there were significant differences in their expression levels in OSF. Furthermore, bioinformatic analysis was performed to explore the roles of these fragments in oral submucous fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 126 tsRNAs in OSF were dysregulated, including 73 upregulated tsRNAs and 53 downregulated tsRNAs. The downregulated tiRNA\u2010Val\u2010CAC\u2010002, tRF\u2010Asn\u2010GTT\u2010005, tRF\u2010Trp\u2010CCA\u2010007 and upregulated tRF\u2010Gly\u2010TCC\u2010016, tRF\u2010Pro\u2010TGG\u2010009\u00a0showed significant differences by qRT\u2010PCR validation, which were consistent with the results of RNA sequencing. Gene ontology and pathway analysis revealed that tRF\u2010Gly\u2010TCC\u2010016 would possibly promote the formation and progress of OSF through cytokine\u2010cytokine receptor interaction and cAMP signal pathway, while tiRNA\u2010Val\u2010CAC\u2010002 could be primarily concerned with the transition from OSF to oral squamous cell carcinoma (OSCC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>tRNA\u2010derived fragments are dysregulated and could be involved in the pathogenesis of oral submucous fibrosis. tRF\u2010Gly\u2010TCC\u2010016 and tiRNA\u2010Val\u2010CAC\u2010002\u00a0may be new regulatory molecules that could affect the process of OSF by regulating signal pathways through interacting with multiple genes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13245","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T09:02:35Z","timestamp":1632474155000},"page":"1057-1066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression profiles of tRNA\u2010derived small RNA and their potential roles in oral submucous fibrosis"],"prefix":"10.1111","volume":"50","author":[{"given":"Liujun","family":"Zeng","sequence":"first","affiliation":[{"name":"Centre of Stomatology Xiangya Hospital Central South University Changsha China"},{"name":"Research Center of Oral and Maxillofacial Tumor Xiangya Hospital Central South University Changsha China"},{"name":"Institute of Oral Cancer and Precancerous Lesions Central South University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Peng","sequence":"additional","affiliation":[{"name":"Centre of Stomatology Xiangya Hospital Central South University Changsha China"},{"name":"Research Center of Oral and Maxillofacial Tumor Xiangya Hospital Central South University Changsha China"},{"name":"Institute of Oral Cancer and Precancerous Lesions Central South University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiqiao","family":"Yu","sequence":"additional","affiliation":[{"name":"Centre of Stomatology Xiangya Hospital Central South University Changsha China"},{"name":"Research Center of Oral and Maxillofacial Tumor Xiangya Hospital Central South University Changsha China"},{"name":"Institute of Oral Cancer and Precancerous Lesions Central South University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiming","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center of Oral and Maxillofacial Tumor Xiangya Hospital Central South University Changsha China"},{"name":"Institute of Oral Cancer and Precancerous Lesions Central South 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