{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:39:04Z","timestamp":1779377944886,"version":"3.53.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160445"],"award-info":[{"award-number":["82160445"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vascular adhesion protein\u20101 (VAP\u20101) is believed to play a role in inflammation. Studies have suggested that VAP\u20101\u2010mediated activation of inflammation is dependent on NF\u2010\u03baB, leading to secretion of the interleukin (IL)\u20108; however, no reports have addressed the association between VAP\u20101 and NF\u2010\u03baB\/IL\u20108\u00a0signaling in oral squamous cell carcinoma (OSCC). This study aimed to investigate the role of VAP\u20101 in OSCC and further explore whether VAP\u20101 is involved in the regulation of neutrophil infiltration in the tumor microenvironment (TME).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunochemistry staining was used to observe VAP\u20101 expression. CCK\u20108 and Transwell assays were used to measure cell proliferation, migration, and invasion. OSCC xenograft mouse models were used for in vivo verification of the VAP\u20101 function. The expression of NF\u2010\u03baB and IL\u20108 were determined by qRT\u2010PCR and western blot. ELISA for IL\u20108 was also conducted. The relationship between VAP\u20101 expression and neutrophil infiltration was analyzed by immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>VAP\u20101 was overexpressed in human OSCC tissues. Downregulation of VAP\u20101\u00a0suppressed OSCC cells proliferation, migration, and invasion in vitro and inhibited tumor proliferation and metastasis in vivo. Additionally, downregulation of VAP\u20101 inhibited NF\u2010\u03baB\/IL\u20108\u00a0signaling in vitro and in vivo. VAP\u20101 expression was positively correlated with neutrophil infiltration in human OSCC tissues. Moreover, blocking VAP\u20101 decreased neutrophil infiltration by reducing IL\u20108 production.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>VAP\u20101 downregulation in OSCC suppresses tumor growth and metastasis by inhibiting NF\u2010\u03baB\/IL\u20108\u00a0signaling and reducing neutrophil infiltration in the TME, suggesting that VAP\u20101\u00a0may be a potential therapeutic target for OSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13285","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T22:48:44Z","timestamp":1645051724000},"page":"332-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Downregulation of VAP\u20101 in OSCC suppresses tumor growth and metastasis via NF\u2010\u03baB\/IL\u20108 signaling and reduces neutrophil infiltration"],"prefix":"10.1111","volume":"51","author":[{"given":"Qiongdong","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology Guangxi Medical University  Nanning China"},{"name":"Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction  Nanning China"},{"name":"Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment  Nanning China"},{"name":"Guangxi Clinical Research Center for Craniofacial Deformity  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueru","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology Guangxi Medical University  Nanning China"},{"name":"Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction  Nanning China"},{"name":"Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease 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Guangxi Medical University  Nanning China"},{"name":"Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction  Nanning China"},{"name":"Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment  Nanning China"},{"name":"Guangxi Clinical Research Center for Craniofacial Deformity  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology Guangxi Medical University  Nanning 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