{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:39:50Z","timestamp":1792190390586,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T00:00:00Z","timestamp":1649030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oral submucosal fibrosis (OSF) is a precancerous condition that closely related to the habit of chewing betel nut. The OSF patients of 3%\u201319% may develop cancer, and this probability is increasing year by year. Epigenetics modifications have been reported as part of the pathogenesis of OSF. However, in OSF field, the role and mechanism of arecoline\u2010induced activation of transforming growth factor \u03b2 (TGF\u2010\u03b2) signaling on N6\u2010methyladenosine (<jats:italic>m6A<\/jats:italic>) modification remain unclear. In this study, we investigated the effect and mechanism of arecoline on <jats:italic>m6A<\/jats:italic> modification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MeRIP\u2010Seq and RNA\u2010seq were performed in arecoline\u2010stimulated cells. Quantitative polymerase chain reaction and western blot were performed to detect the expression of <jats:italic>m6A<\/jats:italic> writers and erasers. CCK\u20108 and flow cytometry analyses were performed to measure the cell viability and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>m6A<\/jats:italic> level was increased in OSF tissues compared to normal tissues; arecoline promoted the <jats:italic>m6A<\/jats:italic> methyltransferase Mettl3 and Mettl14 through TGF\u2010\u03b2. MeRIP\u2010seq and RNA\u2010seq analyses found that MYC was the target gene of Mettl14. In addition, Mettl14 silence reversed the effects of arecoline on cell proliferation and apoptosis in Hacat cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TGF\u2010\u03b2\u2010METTL14\u2010<jats:italic>m6A<\/jats:italic>\u2010MYC axis was crucially implicated in arecoline\u2010mediated OSF and may be an effective therapeutic strategy for OSF treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13292","type":"journal-article","created":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T14:57:22Z","timestamp":1649084242000},"page":"474-482","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<scp>N6<\/scp>\u2010methyladenosine modification contributes to arecoline\u2010mediated oral submucosal fibrosis"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5415-633X","authenticated-orcid":false,"given":"Xia","family":"Li","sequence":"first","affiliation":[{"name":"Department of Oral Medicine Foshan Stomatological Hospital, Medical College of Foshan University  Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Stomatology The Second Xiangya Hospital, Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuya","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Foshan Stomatological Hospital, Medical College of Foshan University  Foshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangcong","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Oral Maxillofacial Surgery 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