{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T01:28:01Z","timestamp":1777598881107,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004799","name":"Australian Dental Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004799","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001801","name":"University of Western Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study assessed the efficacy of using oral liquid\u2010based brush cytology (OLBC) coupled with immunostained cytology\u2010derived cell\u2010blocks, quantified using machine\u2010learning, in the diagnosis of oral lichen planus (OLP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Eighty\u2010two patients diagnosed clinically with either OLP or oral lichenoid lesion (OLL) were included. OLBC samples were obtained from all patients before undergoing surgical biopsy. Liquid\u2010based cytology slides and cell\u2010blocks were prepared and assessed by cytomorphology and immunocytochemistry for four antibodies (Ki\u201067, BAX, NF\u2010\u03baB\u2010p65, and AMACR). For comparison purposes, a sub\u2010group of 31 matched surgical biopsy samples were selected randomly and assessed by immunohistochemistry. Patients were categorized according to their definitive diagnoses into OLP, OLL, and clinically lichenoid, but histopathologically dysplastic lesions (OEDL). Machine\u2010learning was utilized to provide automated quantification of positively stained protein expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cytomorphological assessment was associated with an accuracy of 77.27% in the distinction between OLP\/OLL and OEDL. A strong concordance of 92.5% (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u20090.84) of immunostaining patterns was evident between cell\u2010blocks and tissue sections using machine\u2010learning. A diagnostic index using a Ki\u201067\u2010based model was 100% accurate in detecting lichenoid cases with epithelial dysplasia. A BAX\u2010based model demonstrated an accuracy of 92.16%. The accuracy of cytomorphological assessment was greatly improved when it was combined with BAX immunoreactivity (95%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Cell\u2010blocks prepared from OLBC are reliable and minimally\u2010invasive alternatives to surgical biopsies to diagnose OLLs with epithelial dysplasia when combined with Ki\u201067 immunostaining. Machine\u2010learning has a promising role in the automated quantification of immunostained protein expression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13301","type":"journal-article","created":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T02:24:51Z","timestamp":1650680691000},"page":"563-572","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immunoexpression of oral brush biopsy enhances the accuracy of diagnosis for oral lichen planus and lichenoid lesions"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7206-5389","authenticated-orcid":false,"given":"Majdy","family":"Idrees","sequence":"first","affiliation":[{"name":"UWA Dental School The University of Western Australia  Perth Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6978-4972","authenticated-orcid":false,"given":"Kate","family":"Shearston","sequence":"additional","affiliation":[{"name":"UWA Dental School The University of Western Australia  Perth Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1642-6204","authenticated-orcid":false,"given":"Camile S.","family":"Farah","sequence":"additional","affiliation":[{"name":"Australian Centre for Oral Oncology Research and Education  Nedlands Western Australia Australia"},{"name":"Oral, Maxillofacial and Dental Surgery, Fiona Stanley Hospital  Murdoch Western Australia Australia"},{"name":"Anatomical Pathology, Australian Clinical Labs  Subiaco Western Australia Australia"},{"name":"CQ University  Rockhampton Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5951-8280","authenticated-orcid":false,"given":"Omar","family":"Kujan","sequence":"additional","affiliation":[{"name":"UWA Dental School The University of Western Australia  Perth Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13323"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13170"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12306"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12996"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13704"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.26006"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2021.105362"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13993"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12904"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2016.05.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2007.02.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12660"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12759"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12902"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13153"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2018.19.11.3279"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2785"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753\u2010015\u20100145\u2010x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajg.2016.06.003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374\u2010020\u201000514\u20100"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13226"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201049710\u2010z"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.12.014"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108762"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24379"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2018.5971"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.14.e563"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.09.012"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2021.105282"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.13301","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jop.13301","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.13301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T14:27:34Z","timestamp":1692800854000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.13301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,11]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/jop.13301"],"URL":"https:\/\/doi.org\/10.1111\/jop.13301","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JOP.13301\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,11]]},"assertion":[{"value":"2022-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}