{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T07:03:09Z","timestamp":1760598189845},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,10,9]],"date-time":"2022-10-09T00:00:00Z","timestamp":1665273600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009991","name":"Department of Finance of Jilin Province","doi-asserted-by":"publisher","award":["JLSWSRCZX2020\u20100070","JLSWSRCZX2021\u2010062"],"award-info":[{"award-number":["JLSWSRCZX2020\u20100070","JLSWSRCZX2021\u2010062"]}],"id":[{"id":"10.13039\/501100009991","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Primary Sj\u00f6gren's syndrome is characterized by lymphocytic infiltration and dysfunction of exocrine glands. The persistent presence of lymphocytes in these glands is an important cause of injury to glandular epithelial cells. MicroRNAs play an important role in primary Sj\u00f6gren's syndrome and regulate mRNA expression. This study evaluated the expression of microRNA related to lymphocyte infiltration in primary Sj\u00f6gren's syndrome patients so as to find novel diagnostic and therapeutic targets for primary Sj\u00f6gren's syndrome. We also explored the microRNA\u2013mRNA networks related to lymphocyte infiltration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>mRNA\u2010seq and microRNA\u2010seq of peripheral blood mononuclear cells (PBMCs) were performed on samples from five primary Sj\u00f6gren's syndrome patients and five matched healthy controls. Meanwhile, microRNA\u2013mRNA network analysis was also conducted. RT\u2010qPCR was used for validation of differentially expressed RNAs. Immunohistochemistry analysis was used to detect MMP2 expression in labial gland tissue. Hsa\u2010miR\u20103202 mimics\/inhibitor\u2010transfected Jurkat cells were used to measure the effects of hsa\u2010miR\u20103202 on the infiltration potential of T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>mRNA and microRNA sequencing revealed that 84 differentially expressed mRNAs and 49 differentially expressed microRNAs had a mutual regulatory relationship. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that differentially expressed <jats:italic>MMP2<\/jats:italic> and its related microRNA, hsa\u2010miR\u20103202, were enriched in the leukocyte transendothelial migration pathway. MMP2 was highly expressed in PBMC and labial gland tissue in primary Sj\u00f6gren's syndrome patients. Hsa\u2010miR\u20103202 was lower expressed in primary Sj\u00f6gren's syndrome than healthy control. Furthermore, hsa\u2010miR\u20103202 mimics transfection decreased MMP2 expression in Jurkat cells, and inhibited Jurkat cells invasion (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.047).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Large number of differentially expressed microRNAs and mRNAs were detected in primary Sj\u00f6gren's syndrome, and these differentially expressed microRNAs and mRNAs had a mutual regulatory relationship and played an important role in primary Sj\u00f6gren's syndrome. In the study, we found hsa\u2010miR\u20103202 regulate <jats:italic>MMP2<\/jats:italic> and inhibited the infiltration of T cells from peripheral blood into the gland, which played a protective role.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13355","type":"journal-article","created":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T04:33:20Z","timestamp":1662698000000},"page":"818-828","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>Hsa\u2010miR<\/scp>\u20103202 attenuates Jurkat cell infiltration via <scp><i>MMP2<\/i><\/scp> in primary Sj\u00f6gren's syndrome"],"prefix":"10.1111","volume":"51","author":[{"given":"Qi","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology The First Hospital of Jilin University  Changchun 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