{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T22:25:47Z","timestamp":1782167147676,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008532","name":"Universidade Federal do Rio Grande do Norte","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008532","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Epithelial\u2013mesenchymal transition is one of the main mechanisms for tumor progression and metastasis. Transcription factors such as TWIST1 are key regulators of the epithelial\u2013mesenchymal transition and are regarded as potential therapeutic targets for the treatment of cancer. The purpose of this study was to examine TWIST1 as a possible epithelial\u2010mesenchymal transition\u2010related prognostic biomarker in oral epithelial dysplasia and oral tongue squamous cell carcinomas, as well as the biological behavior of TWIST1\u2010silencing in oral tongue squamous cell carcinomas cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunohistochemical analysis of TWIST1, E\u2010cadherin, and N\u2010cadherin was carried out in 47 samples representing oral epithelial dysplasia and 41 oral tongue squamous cell carcinomas. The suppression of TWIST1 expression was performed using shRNA\u2010expression vectors in HSC\u20103 and SCC\u20109 cells to investigate in vitro the impact of TWIST1 on proliferation, apoptosis, viability, migration, and invasion of SCC\u20109 and HSC\u20103 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The expression of nuclear TWIST1 was significantly higher in oral tongue squamous cell carcinomas than in oral epithelial dysplasis (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), whereas TWIST1 in the cytoplasm was more expressed in oral epithelial dysplasis (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.012). The high cytoplasmic expression of TWIST1 was significantly associated with shortened overall survival (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), and increased nuclear TWIST1 expression was significantly related to high risk of recurrence (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.03). Knockdown of TWIST1 in oral tongue squamous cell carcinomas cells induced the expression of E\u2010cadherin and inhibited N\u2010cadherin, which were followed by decreased proliferation, migration, and invasion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our research suggests that TWIST1 is linked to the development of oral tongue carcinogenesis and may be used as a prognostic indicator and therapeutic target for oral tongue squamous cell carcinomas patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13377","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T04:50:34Z","timestamp":1666759834000},"page":"127-135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>TWIST1<\/scp>\n                    regulates proliferation, migration, and invasion and is a prognostic marker for oral tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2173-7672","authenticated-orcid":false,"given":"Everton Freitas","family":"de Morais","sequence":"first","affiliation":[{"name":"Federal University of Rio Grande do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3488-3465","authenticated-orcid":false,"given":"Hannah Gil","family":"de Farias Morais","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edilmar","family":"de Moura Santos","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1979-9919","authenticated-orcid":false,"given":"Carlos Augusto Galv\u00e3o","family":"Barboza","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7608-0738","authenticated-orcid":false,"given":"F\u00e1bio Haach","family":"T\u00e9o","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, School of Dentistry University of Campinas (UNICAMP)  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6039-0088","authenticated-orcid":false,"given":"Tuula","family":"Salo","sequence":"additional","affiliation":[{"name":"Cancer and Translational Medicine Research Unit, Faculty of Medicine and Medical Research Center Oulu Oulu University Hospital, University of Oulu  Oulu Finland"},{"name":"Department of Pathology, Helsinki University Hospital Institute of Oral and Maxillofacial Disease, University of Helsinki, and HUSLAB  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5285-3046","authenticated-orcid":false,"given":"Ricardo D.","family":"Coletta","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis, School of Dentistry University of Campinas (UNICAMP)  Piracicaba Brazil"},{"name":"School of Dentistry University of Campinas  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7577-5375","authenticated-orcid":false,"given":"Roseana","family":"de Almeida Freitas","sequence":"additional","affiliation":[{"name":"Federal University of Rio Grande do Norte  Natal 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