{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:19Z","timestamp":1772160739034,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["22ZR1437000"],"award-info":[{"award-number":["22ZR1437000"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Soluble E\u2010cadherin (sEcad), a tumor suppressor gene, has pro\u2010oncogenic effects by binding to human epithelial growth factor receptor 2 (\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      ). In our previous study, 1\/3 of carcinoma ex pleomorphic adenoma (CXPA) cases had\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      amplification, which is associated with tumorigenesis and malignancy. This study examines the role of sEcad in\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      amplified CXPA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Immunohistochemistry was used to examine E\u2010cadherin (Ecad) expression in\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      \u2010amplified CXPA samples (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a035). Western blot and ELISA were used to detect sEcad in two samples with Ecad and\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      overexpression and CXPA cell line. Lentivirus\u2010mediated transfection was performed to knock down sEcad in CXPA cells. The cell proliferation, wound healing, and transwell assays were used to compare sEcad\u2010knockdown cells with cells pretreated with recombinant human sEcad (rhEcad\/Fc). sEcad and\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      interaction was determined through co\u2010immunoprecipitation. RNA\u2010sequencing, differential expression analysis, GO and KEGG analysis were used to identify sEcad\n                      <jats:italic>\u2010<\/jats:italic>\n                      related signaling pathways and their protein phosphorylation levels were verified by western blotting.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Ecad was overexpressed in 77.1% of\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      \u2010positive CXPA, and sEcad was found in the CXPA cell line and two samples. sEcad promoted CXPA migration and invasion in vitro without sEcad and\n                      <jats:italic>HER\u20102<\/jats:italic>\n                      interaction. sEcad\u2010related differentially expressed genes were enriched in the IL\u201017, cAMP, and MAPK signaling pathways. Furthermore, sEcad activated the phosphorylation of Akt and MAPK\/ERK signaling pathways.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Most HER\u20102\n                      <jats:sup>+<\/jats:sup>\n                      CXPAs express Ecad. sEcad could affect the invasiveness and migration of in vitro CXPA cells without\n                      <jats:italic>HER\u20102.<\/jats:italic>\n                      sEcad may be a therapeutic biomarker for CXPA patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13388","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T10:19:51Z","timestamp":1669717191000},"page":"63-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Soluble E\u2010cadherin promotes invasiveness of neoplastic cells in salivary gland carcinoma ex pleomorphic adenoma"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0357-5566","authenticated-orcid":false,"given":"Wanling","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Oral Pathology Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sai","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"},{"name":"Department of Oral Mucosal Diseases Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoning","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajun","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"},{"name":"Department of Oral and Craniomaxillofacial Surgery Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai 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