{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T03:06:56Z","timestamp":1777000016894,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Respiratory epithelial adenomatoid hamartoma (REAH) is a sinonasal glandular overgrowth arising from the surface respiratory epithelium and invaginating into the stroma. Clinically, it appears as a polypoid mass that may cause nasal obstruction, anosmia, and epistaxis. The presence of cartilaginous and\/or osseous areas move the lesion to a chondro\u2010osseous respiratory epithelial (CORE) hamartoma subtype. Scattered small seromucinous glands may be observed between typical REAH glands and when it is the only feature, it represents seromucinous hamartoma (SH). The molecular pathogenesis of REAH has been poorly explored and remains unclear. Given that <jats:italic>KRAS<\/jats:italic>, <jats:italic>BRAF<\/jats:italic>, and <jats:italic>EGFR<\/jats:italic> mutations have been detected in a variety of sinonasal tumors, we aimed to assess these mutations in REAH and SH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ten REAH (including one CORE subtype), in addition to two SH cases, were Sanger sequenced by standard techniques. The targeted regions included <jats:italic>KRAS<\/jats:italic> exons 2\u20134 (encompassing hotspots codons 12, 13, 61, and 146), <jats:italic>BRAF<\/jats:italic> exons 11 and 15 (spanning the V600 codon), and <jats:italic>EGFR<\/jats:italic> exons 19 and 20.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All REAH and SH samples showed wild\u2010type sequences for <jats:italic>KRAS<\/jats:italic>, <jats:italic>BRAF<\/jats:italic>, and <jats:italic>EGFR<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate a lack of <jats:italic>KRAS<\/jats:italic>, <jats:italic>BRAF<\/jats:italic>, or <jats:italic>EGFR<\/jats:italic> pathogenic variants with further evaluation of REAH and SH needed to elucidate driver genetic events.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13394","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T06:44:26Z","timestamp":1670827466000},"page":"548-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of <i>KRAS<\/i>, <i>BRAF<\/i>, and <i>EGFR<\/i> mutational status in respiratory epithelial adenomatoid hamartoma (<scp>REAH<\/scp>)"],"prefix":"10.1111","volume":"52","author":[{"given":"Let\u00edcia Martins","family":"Guimar\u00e3es","sequence":"first","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara da Silva","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Armando","family":"De Marco","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lester D. R.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Head and Neck Pathology Consultations  Woodland Hills California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1580-4995","authenticated-orcid":false,"given":"Carolina Cavalieri","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,21]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"WHO Classification of Tumours Editorial Board. Head and Neck Tumours","author":"Bullock J","year":"2022"},{"key":"e_1_2_10_3_1","volume-title":"WHO Classification of Tumours Editorial Board. 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