{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T18:48:27Z","timestamp":1778006907006,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T00:00:00Z","timestamp":1689120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Odontogenic keratocysts constitute 10%\u201320% of odontogenic cysts and exhibit a distinctive corrugated parakeratinized lining epithelium. Considering that cornified envelope formation is an important phenomenon during keratinocyte differentiation, this study aimed to clarify the characteristics of cornified envelope formation in odontogenic keratocysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated the cellular distribution of cornified envelope\u2010related proteins (transglutaminases and their substrates), as well as the upstream regulatory protein c\u2010Fos, by immunohistochemical analysis of the lining epithelium of 20 odontogenic keratocysts. We examined the corresponding mRNA levels by quantitative polymerase chain reaction. Ten dentigerous cysts served as control non\u2010keratinized cysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The distributions of transglutaminase and their substrates except loricrin and small protein\u2010rich protein 1a significantly differed between odontogenic keratocysts and dentigerous cysts. There was no significant difference in c\u2010Fos expression between odontogenic keratocysts and dentigerous cysts. The mRNA levels of transglutaminases and their substrates were significantly higher in odontogenic keratocysts than in dentigerous cysts. However, c\u2010Fos mRNA levels did not significantly differ between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Surprisingly, the overall appearance of cornified envelope\u2010related proteins of odontogenic keratocysts was consistent with the characteristics of non\u2010keratinized oral mucosa identified in previous studies. These findings indicate that the contribution of cornified envelope\u2010related molecules in odontogenic keratocysts is similar to that in non\u2010keratinized oral epithelium, rather than keratinized oral epithelium, suggesting that odontogenic keratocysts are not genuine keratinized cysts. The upregulation of cornified envelope\u2010related genes in odontogenic epithelium could be an important pathognomonic event during odontogenic keratocyst development.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13464","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T05:04:29Z","timestamp":1689224669000},"page":"758-765","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comprehensive cornified envelope protein profile of odontogenic keratocysts clarifies the characteristics of non\u2010keratinized oral epithelium"],"prefix":"10.1111","volume":"52","author":[{"given":"Rita R.","family":"Roy","sequence":"first","affiliation":[{"name":"Department of physiology Matsumoto Dental University  Shiojiri Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takanaga","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, Division of Oral Pathogenesis &amp; Disease Control Asahi University School of Dentistry  Mizuho Japan"},{"name":"Department of Pathology Matsumoto Dental University  Shiojiri Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumitsu","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Pathology Matsumoto Dental University  Shiojiri Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8222-9658","authenticated-orcid":false,"given":"Hiromasa","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Shinshu University Hospital  Matsumoto Japan"},{"name":"Hard Tissue Pathology Unit, Graduate School of Oral Medicine Matsumoto Dental University  Shiojiri Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,12]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"WHO Classification of Head and Neck Tumours","author":"El\u2010Naggar AK","year":"2017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.2.693"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(77)90059\u20109"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0014\u20105793(00)01806\u20108"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.16.11013"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.90.3.732"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0552"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12760"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523\u20101747.1998.00071.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2010.05.007"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795\u2010013\u20100058\u20104"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100714.1993.tb00117.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.244"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601\u20100825.2009.01590.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12418"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.30.18026"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520290903039078"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060518798261"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.3.2021"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523\u20101747.ep12606176"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102133.2003.05288.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0923\u20101811(03)00091\u20104"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780600589065"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383\u2010009\u20102517\u20105"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.4103\/2141\u20109248.153621"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20107580.2009.01066.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100722.2000.00717.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00918.2011"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.24955"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.080042"}],"container-title":["Journal of Oral Pathology &amp; 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