{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:52:12Z","timestamp":1783461132779,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Salivary gland pleomorphic adenoma (SPA) is a common neoplasm of salivary glands that displays remarkable histological diversity. Previous studies have demonstrated the involvement of gene rearrangements and cytoskeleton\u2010remodeling\u2010related myoepithelial cells in SPA tumorigenesis. Cytoskeleton remodeling is necessary for epithelial\u2010mesenchymal transition (EMT), a key process in tumor progression. However, the heterogeneity of tumor cells and cytoskeleton remodeling in SPA has not been extensively investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An analysis of single\u2010cell RNA sequencing (scRNA\u2010seq) was performed on 27\u2009810 cells from two donors with SPA. Bioinformatic tools were used to assess differentially expressed genes, cell trajectories, and intercellular communications. Immunohistochemistry and double immunofluorescence staining were used to demonstrate FOXC1 and MYLK expression in SPA tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our analysis revealed five distinct cell subtypes within the tumor cells of SPA, indicating a high level of intra\u2010lesional heterogeneity. Cytoskeleton\u2010remodeling\u2010related genes were highly enriched in subtype 3 of the tumor cells, which showed a close interaction with mesenchymal cells. We found that tumoral FOXC1 expression was closely related to MYLK expression in the tumor cells of SPA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Tumor cells enriched with cytoskeleton\u2010remodeling\u2010related genes play a crucial role in SPA development, and FOXC1 may partially regulate this process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13465","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T16:41:03Z","timestamp":1691426463000},"page":"766-776","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single\u2010cell <scp>RNA<\/scp> sequencing reveals tumor heterogeneity within salivary gland pleomorphic adenoma: A preliminary study"],"prefix":"10.1111","volume":"52","author":[{"given":"Xi\u2010Qian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Head Neck Surgery, Henan Provincial People's Hospital Zhengzhou University People's Hospital, Henan University People's Hospital  Zhengzhou Henan China"},{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian\u2010Nian","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-2086","authenticated-orcid":false,"given":"Qi\u2010Wen","family":"Man","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Oral and Maxillofacial \u2010 Head Neck Oncology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang\u2010Cai","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Head Neck Surgery, Henan Provincial People's Hospital Zhengzhou University People's Hospital, Henan University People's Hospital  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010Chen","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Head Neck Surgery, Henan Provincial People's Hospital Zhengzhou University People's Hospital, Henan University People's Hospital  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Wei","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Head Neck Surgery, Henan Provincial People's Hospital Zhengzhou University People's Hospital, Henan University People's Hospital  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Gong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Head Neck Surgery, Henan Provincial People's Hospital Zhengzhou University People's Hospital, Henan University People's Hospital  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2024-6353","authenticated-orcid":false,"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; 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