{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:00:35Z","timestamp":1773928835968,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31370943"],"award-info":[{"award-number":["31370943"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970921"],"award-info":[{"award-number":["81970921"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oral breathing has an important impact on morphology and bone mineral density (BMD) in a mandible. This study aimed to investigate the hub genes and mechanism regulating the mandibular BMD decrease induced by nasal obstruction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A unilateral nasal obstruction model was established in 1\u2010week\u2010old Wistar rats by electrocautery obstruction. BMD of the mandible was determined by micro\u2010computed tomography. Transcriptome analysis was performed to identify differentially expressed genes (DEGs). Hub genes were identified by building protein\u2013protein interaction network and verified by western blot. A hypoxic cell model was established in bone marrow mesenchymal stem cells (BMSCs) by using CoCl2. The expression of hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1), NF\u2010kB ligand\u2013receptor activator (RANKL), osteoprotegerin (OPG), and Cyp1a1 was detected by western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mandibular BMD of rats in the unilateral nasal obstruction group was significantly decreased. A total of 38 DEGs were identified in nasal obstruction rats compared with normal rats. A ratio of RANKL\/OPG in the mandible was elevated by nasal obstruction, while the Cyp1a1 was decreased. In vitro, the HIF\u20101\u03b1 expression and RANKL\/OPG ratio were upregulated by hypoxia while the Cyp1a1 expression was decreased. Pretreatment with Cyp1a1 activator, FICZ, could increase the expression of Cyp1a1 while attenuating the activation of HIF\u20101\u03b1 and RANKL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Respiratory changes caused by nasal obstruction contribute to the decrease in Cyp1a1 expression in the mandible of juvenile rats, which is associated with disturbances in bone homeostasis controlled by the RANKL\/OPG ratio.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13468","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T07:58:08Z","timestamp":1690271888000},"page":"786-794","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hypoxia caused by unilateral nasal obstruction decreases mandibular density in rats through inhibition of <scp>Cyp1a1<\/scp> expression"],"prefix":"10.1111","volume":"52","author":[{"given":"Yixin","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Orthodontic, Stomatological Hospital Southern Medical University  Guangzhou Guangdong Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, School and Hospital of Stomatology Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"184","article-title":"Pediatric obstructive sleep apnea and the critical role of oral\u2010facial growth: evidences","volume":"3","author":"Huang YS","year":"2012","journal-title":"Front Neurol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ocr.12220"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/NSS.S288618"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/2176-9451.20.4.039-044.oar"},{"issue":"6","key":"e_1_2_9_6_1","first-page":"311","article-title":"Alveolar bone density reduction in rats caused by unilateral nasal obstruction","volume":"36","author":"Wang X","year":"2019","journal-title":"Balkan Med J"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jds.2021.05.012"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1074"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.51286"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389200219666180529112913"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5591251"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-07-2110"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-0430"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.18240\/ijo.2022.03.04"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1081\/DMR-120033996"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2016.05.022"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.11138\/ccmbm\/2017.14.1.209"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2RA00622G"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI137560"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R400004200"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.2011.586630"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13166"}],"container-title":["Journal of Oral Pathology &amp; 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