{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:13:37Z","timestamp":1785932017235,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,7]],"date-time":"2024-01-07T00:00:00Z","timestamp":1704585600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ameloblastoma is an aggressively growing, highly recurrent odontogenic jaw tumor. Its association with BRAFV600E mutation is an indication for BRAFV00E\u2010inhibitor therapy The study objective was to identify a sensitive low\u2010cost test for BRAFV600E\u2010positive ameloblastoma. We hypothesized that immunohistochemical staining of formalin\u2010fixed paraffin\u2010embedded tissues for BRAFV600E mutation is a low\u2010cost surrogate for BRAFV600E gene sequencing when laboratory resources are inadequate for molecular testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tissues from 40 ameloblastoma samples were retrieved from either formalin\u2010fixed paraffin\u2010embedded blocks, RNAlater\u2122 stabilization solution or samples inadvertently pre\u2010fixed in formalin before transfer to RNAlater\u2122. BRAFV600E mutation was assessed by Direct Sanger sequencing, Amplification Refractory Mutation System\u2010PCR and immunohistochemistry (IHC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>BRAFV600E mutation was detected by IHC, Amplification Refractory Mutation System\u2010PCR and Direct Sanger sequencing in 93.33%, 52.5% and 30% of samples respectively. Considering Direct Sanger sequencing as standard BRAFV600E detection method, there was significant difference between the three detection methods (\ud835\udf12<jats:sup>2<\/jats:sup> (2)\u2009=\u200931.34, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Sensitivity and specificity of IHC were 0.8 (95% CI: 0.64\u20130.90) and 0.9 (95% CI: 0.75\u20130.99) respectively, while positive predictive value and negative predictive value (NPV) were 0.9 and 0.8 (Fischer's test, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) respectively. Sensitivity and specificity of Amplification Refractory Mutation System\u2010PCR detection method were 0.7 (95% CI: 0.53\u20130.80) and 0.9 (95% CI\u2009=\u20090.67\u20130.98) respectively, while PPV and NPV were 0.9 and 0.6 respectively (Fischer's test, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Low\u2010cost and less vulnerability of IHC to tissue quality make it a viable surrogate test for BRAFV600E detection in ameloblastoma. Sequential dual IHC and molecular testing for BRAFV600E will reduce equivocal results that could exclude some patients from BRAFV600E\u2010inhibitor therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13506","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:48:45Z","timestamp":1704674925000},"page":"79-87","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of diagnostic methods for detection of <scp>BRAFV600E<\/scp> mutation in ameloblastoma"],"prefix":"10.1111","volume":"53","author":[{"given":"Arularasan","family":"Anbinselvam","sequence":"first","affiliation":[{"name":"Department of Oral Medicine, School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1182-107X","authenticated-orcid":false,"given":"Abdul\u2010Warith O.","family":"Akinshipo","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology\/Biology, Faculty of Dental Sciences University of Lagos  Lagos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4615-2264","authenticated-orcid":false,"given":"Akinyele O.","family":"Adisa","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology University of Ibadan and University College Hospital Ibadan  Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8678-4966","authenticated-orcid":false,"given":"Olajumoke A.","family":"Effiom","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology\/Biology, Faculty of Dental Sciences University of Lagos  Lagos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhe","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine, School of Dental Medicine University of Pennsylvania  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0747-1513","authenticated-orcid":false,"given":"Kehinde E.","family":"Adebiyi","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology &amp; 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