{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T19:46:42Z","timestamp":1784404002454,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2024,5]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n To evaluate the presence of myofibroblasts (MFs) in the development of lip carcinogenesis, through the correlation of clinical, histomorphometric and immunohistochemical parameters, in actinic cheilitis (ACs) and lower lip squamous cell carcinomas (LLSCCs).<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Samples of ACs, LLSCCs, and control group (CG) were prepared by tissue microarray (TMA) for immunohistochemical TGF\u2010\u03b2, \u03b1\u2010SMA, and Ki\u201067 and histochemical hematoxylin and eosin, picrosirius red, and verhoeff van gieson reactions. Clinical and microscopic data were associated using the Mann\u2013Whitney, Kruskal\u2010Wallis\/Dunn, and Spearman correlation tests (SPSS,\n p<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n ACs showed higher number of \u03b1\u2010SMA\n +<\/jats:sup>\n MFs when compared to CG (\n p<\/jats:italic>\n \u2009=\u20090.034), and these cells were associated with the vertical expansion of solar elastosis (SE) itself (\n p<\/jats:italic>\n \u2009=\u20090.027). Areas of SE had lower deposits of collagen (\n p<\/jats:italic>\n \u2009<\u20090.001), immunostaining for TGF\u2010\u03b2 (\n p<\/jats:italic>\n \u2009<\u20090.001), and higher density of elastic fibers (\n p<\/jats:italic>\n \u2009<\u20090.05) when compared to areas without SE. A positive correlation was observed between high\u2010risk epithelial dysplasia (ED) and the proximity of SE to the dysplastic epithelium (\n p<\/jats:italic>\n \u2009=\u20090.027). LLSCCs showed a higher number of \u03b1\u2010SMA\n +<\/jats:sup>\n MFs about CG (\n p<\/jats:italic>\n \u2009=\u20090.034), as well as a reduction in the deposition of total collagen (\n p<\/jats:italic>\n \u2009=\u20090.009) in relation to ACs and CG. There was also a negative correlation between the amount of \u03b1\u2010SMA\n +<\/jats:sup>\n cells and the accumulation of total collagen (\n p<\/jats:italic>\n \u2009=\u20090.041). Collagen and elastic density loss was higher in larger tumors (\n p<\/jats:italic>\n \u2009=\u20090.045) with nodal invasion (\n p<\/jats:italic>\n \u2009=\u20090.047).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Our findings show the possible role of MFs, collagen fibers, and elastosis areas in the lip carcinogenesis process.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.13541","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:14:09Z","timestamp":1714608849000},"page":"321-330","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma"],"prefix":"10.1111","volume":"53","author":[{"given":"Farah Essgui Orellana","family":"Martinez","sequence":"first","affiliation":[{"name":"Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing Federal University of Cear\u00e1 (UFC) Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Th\u00e2mara Manoela Marinho","family":"Bezerra","sequence":"additional","affiliation":[{"name":"Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing Federal University of Cear\u00e1 (UFC) Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Negreiros Nunes","family":"Alves","sequence":"additional","affiliation":[{"name":"Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing Federal University of Cear\u00e1 (UFC) Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle Joyce Lima Silva","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Cear\u00e1 School of Oncology Haroldo Jua\u00e7aba Hospital Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabricio Bitu","family":"Sousa","sequence":"additional","affiliation":[{"name":"Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing Federal University of Cear\u00e1 (UFC) Fortaleza Brazil"},{"name":"Department of Dentistry Christus University Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Goberl\u00e2nio","family":"de Barros Silva","sequence":"additional","affiliation":[{"name":"Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing Federal University of Cear\u00e1 (UFC) Fortaleza Brazil"},{"name":"Department of Dentistry Christus University Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio Rog\u00e9rio Lima","family":"Mota","sequence":"additional","affiliation":[{"name":"Division of Oral Pathology, Department of Dental Clinic, Faculty of Pharmacy, Dentistry and Nursing Federal University of Cear\u00e1 (UFC) Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4067\/s0717\u201095022012000200044"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2022.105387"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970\u20109290.74224"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2020.104972"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12623"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.239"},{"key":"e_1_2_11_8_1","first-page":"1","article-title":"Transforming growth 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