{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:35:36Z","timestamp":1790800536652,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T00:00:00Z","timestamp":1718150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270983"],"award-info":[{"award-number":["82270983"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oral lichen planus (OLP) is a common T cell\u2010mediated oral mucosal immune inflammatory disease. Intraepithelial lymphocytes (IELs) are a unique subset of T cells that play an important role in regulating immune response. This study aims to investigate the phenotype and the differentiation mechanism of IELs in OLP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of CD4, CD8\u03b1, CD8\u03b2, T\u2010helper\u2010inducing POZ\/Krueppel\u2010like factor (ThPOK), and RUNX family transcription factor 3 (Runx3) in the epithelium and peripheral blood mononuclear cells (PBMCs) of OLP was determined by immunofluorescence and immunohistochemistry. Then, the correlations among them were analyzed. Na\u00efve CD4<jats:sup>+<\/jats:sup> T cells were sorted from blood of OLP patients and stimulated with retinoic acid (RA) and transforming growth factor\u2010\u03b2<jats:sub>1<\/jats:sub> (TGF\u2010\u03b2<jats:sub>1<\/jats:sub>). Then the expression of CD4, CD8\u03b1, CD8\u03b2, ThPOK, and Runx3 was investigated by immunocytochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD8\u03b1 expression and CD8\u03b1\u03b1<jats:sup>+<\/jats:sup> cells were upregulated in the epithelium of OLP, whereas they were downregulated in PBMCs of OLP. CD8\u03b2 was not expressed in the epithelium of OLP. CD4, CD8\u03b1, and Runx3 expression and CD4<jats:sup>+<\/jats:sup>CD8\u03b1<jats:sup>+<\/jats:sup> cells were increased, whereas ThPOK expression was decreased in the epithelium of OLP. CD8\u03b1 expression was positively correlated with Runx3 expression, whereas ThPOK expression was negatively correlated with Runx3 expression. After RA and TGF\u2010\u03b2<jats:sub>1<\/jats:sub> stimulation, CD8\u03b1 and Runx3 expression was upregulated, and ThPOK expression was downregulated in na\u00efve CD4<jats:sup>+<\/jats:sup> T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CD4<jats:sup>+<\/jats:sup>CD8\u03b1\u03b1<jats:sup>+<\/jats:sup> IELs may be the dominant phenotype of IELs in OLP, and the differentiation of CD4<jats:sup>+<\/jats:sup>CD8\u03b1\u03b1<jats:sup>+<\/jats:sup> IELs in OLP is negatively regulated by ThPOK and positively regulated by Runx3.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.13564","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:35:16Z","timestamp":1718238916000},"page":"480-490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>CD4<\/scp><sup>+<\/sup><scp>CD8\u03b1\u03b1<\/scp><sup>+<\/sup> is the dominant phenotype of intraepithelial lymphocytes and regulated by <scp>ThPOK<\/scp> and Runx3 in oral lichen planus"],"prefix":"10.1111","volume":"53","author":[{"given":"Chao\u2010Fan","family":"Bao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4988-0814","authenticated-orcid":false,"given":"Gang","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral &amp; Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Oral Medicine, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2020.151933"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16964"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1701704"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.3ru0220\u2010111"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.05.073"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40495\u2010017\u20100105\u20102"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590\u2010021\u201000980\u20108"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2523"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.11.003"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2016.05.004"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281\u2010012\u20100316\u2010x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326\u20106066.Cir\u201022\u20100041"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102613.2012.00827.x"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.2021039957"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3RU1014\u2010465R"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14142"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16935"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102567.2005.02235.x"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00557"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102172\u20108\u201012"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11030955"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24033038"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102006\u201008\u2010038844"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1997"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1652"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151844"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010018\u20100013\u20107"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02883"}],"container-title":["Journal of Oral Pathology &amp; 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