{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T12:49:21Z","timestamp":1793364561607,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T00:00:00Z","timestamp":1729641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018219","name":"Hospital de C\u00e2ncer de Barretos","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2024,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n The role of microbiome, particularly\n Fusobacterium nucleatum<\/jats:italic>\n (\n Fn<\/jats:italic>\n ), in periodontal disease and oral squamous cell carcinoma (OSCC) has been recently explored. This study aimed to evaluate the\n Fn<\/jats:italic>\n presence and its levels in oral rinse samples from Brazilian OSCC patients and healthy individuals and its association with sociodemographic, clinical, and oral health features.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this case\u2013control study, 80 participants were included, 31 OSCC patients and 49 individuals without a cancer history. Clinical data were collected, and an oral exam was done on a subset of the cohort.\n Fn<\/jats:italic>\n levels were evaluated by droplet digital PCR (ddPCR) in oral rinse samples and were categorized as\n Fn\u2010<\/jats:italic>\n high or\n Fn<\/jats:italic>\n \u2010low based on the median number of copies per reaction.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n OSCC patients showed higher levels of\n Fn<\/jats:italic>\n (68%,\n p<\/jats:italic>\n \u2009=\u20090.03) than controls, and all OSCC cases were diagnosed with periodontal disease (100%,\n p<\/jats:italic>\n \u2009=\u20091.0). In the univariate analysis,\n Fn\u2010<\/jats:italic>\n high level was more frequently present in OSCC cases compared to controls (\n p<\/jats:italic>\n \u2009=\u20090.01). It was also observed that\n Fn\u2010<\/jats:italic>\n high level OSCC cases were significantly associated with self\u2010reported non\u2010white ethnicity (71.4%,\n p<\/jats:italic>\n \u2009=\u20090.01) and had more infiltrative lesions (57.1%,\n p<\/jats:italic>\n \u2009=\u20090.02) than\n Fn<\/jats:italic>\n \u2010low OSCC cases.\n Fn<\/jats:italic>\n \u2010high levels in oral rinse samples, were significantly more prevalent among OSCC than in controls.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n In OSCC patients,\n Fn\u2010<\/jats:italic>\n high levels were associated with non\u2010white ethnicity and lesions with infiltrative clinical aspects. Among OSCC cases, all had periodontal disease.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.13580","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T01:40:25Z","timestamp":1729734025000},"page":"657-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Association of\n Fusobacterium nucleatum<\/i>\n Levels by\n ddPCR<\/scp>\n in Oral Rinse Samples With Periodontal Disease in Oral Squamous Cell Carcinoma Patients and in Controls"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4419-6944","authenticated-orcid":false,"given":"B\u00e1rbara Borella","family":"Fernandes","sequence":"first","affiliation":[{"name":"Department of Oral Cancer Prevention Barretos Cancer Hospital Barretos Brazil"},{"name":"Department of Dentistry Barretos Cancer Hospital Barretos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Guilherme","family":"Datorre","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center Barretos Cancer Hospital Barretos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana de Lima","family":"Vazquez","sequence":"additional","affiliation":[{"name":"Department of Oral Cancer Prevention Barretos Cancer Hospital Barretos Brazil"},{"name":"Department of Dentistry Barretos Cancer Hospital Barretos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina de Carvalho","family":"Peters","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center Barretos Cancer Hospital Barretos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio Luiz","family":"Coracin","sequence":"additional","affiliation":[{"name":"Department of Oral Cancer Prevention Barretos Cancer Hospital Barretos Brazil"},{"name":"Department of Dentistry Barretos Cancer Hospital Barretos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9639-7940","authenticated-orcid":false,"given":"Rui Manuel","family":"Reis","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center Barretos Cancer Hospital Barretos Brazil"},{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine University of Minho Braga Portugal"},{"name":"ICVS\/3B's\u2010PT Government Associate Laboratory Braga\/Guimar\u00e3es Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia Maria Rebolho Batista","family":"Arantes","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center Barretos Cancer Hospital Barretos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Ribeiro","family":"Gama","sequence":"additional","affiliation":[{"name":"Head and Neck Surgery Department Barretos Cancer Hospital Barretos 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