{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:15:41Z","timestamp":1790255741991,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,11,24]],"date-time":"2024-11-24T00:00:00Z","timestamp":1732406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Oropharyngeal squamous cell carcinoma (OP\u2010SCC) represents a public health problem and human papillomavirus (HPV) is one of the risk factors. Neutrophil extracellular traps (NET) are meshes of DNA strands and granule proteins. NET has been identified in diverse cancers, whether associated with viruses or not. However, there is no information on NET in OP\u2010SCC. We aimed to evaluate the NET release by neutrophils in the OP\u2010SCC microenvironment, stratified by HPV status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This cross\u2010sectional study analyzed OP\u2010SCC biopsy specimens diagnosed from 1997 to 2021. HPV status was determined by p16 immunohistochemistry and \u201cin situ\u201d hybridization. Neutrophils were detected by CD66b immunohistochemistry. Immunofluorescence was used to identify NET by co\u2010localization of myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit). Bivariate statistics, Kaplan\u2013Meier survival analysis, and the log\u2010rank test were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HPV\u2010positive and HPV\u2010negative OP\u2010SCC had similar CD66b\u2009+\u2009neutrophil infiltration (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05), but the release of NET was significantly increased in HPV\u2010positive compared to HPV\u2010negative OP\u2010SCC samples (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Overall survival was not impacted by NET indexes (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The presence of HPV may stimulate NET release in the OP\u2010SCC microenvironment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13594","type":"journal-article","created":{"date-parts":[[2024,11,24]],"date-time":"2024-11-24T20:34:23Z","timestamp":1732480463000},"page":"57-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Contribution of\n                    <scp>HPV<\/scp>\n                    Status for Neutrophil Extracellular Traps Release in Oropharyngeal Cancer"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7655-8666","authenticated-orcid":false,"given":"Adriana Aparecida Silva","family":"da Costa","sequence":"first","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais (UFMG). Av. Ant\u00f4nio Carlos  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sic\u00edlia Rezende","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais (UFMG). Av. Ant\u00f4nio Carlos  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7220-1360","authenticated-orcid":false,"given":"Thalita Soares","family":"Tavares","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais (UFMG). Av. Ant\u00f4nio Carlos  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Pereira","family":"Meirelles","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais (UFMG). Av. Ant\u00f4nio Carlos  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ev\u00e2nio Vilela","family":"da Silva","sequence":"additional","affiliation":[{"name":"Oral Pathology, Department of Stomatology, Public Oral Health, and Forensic Dentistry, School of Dentistry Universidade de S\u00e3o Paulo (USP). Av. do Caf\u00e9 \u2010 Subsetor Oeste  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson Tangerino Ferreira","family":"da Silva","sequence":"additional","affiliation":[{"name":"Oral Pathology, Department of Stomatology, Public Oral Health, and Forensic Dentistry, School of Dentistry Universidade de S\u00e3o Paulo (USP). Av. do Caf\u00e9 \u2010 Subsetor Oeste  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9668-5870","authenticated-orcid":false,"given":"Jorge Esquiche","family":"Le\u00f3n","sequence":"additional","affiliation":[{"name":"Oral Pathology, Department of Stomatology, Public Oral Health, and Forensic Dentistry, School of Dentistry Universidade de S\u00e3o Paulo (USP). Av. do Caf\u00e9 \u2010 Subsetor Oeste  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1809-0617","authenticated-orcid":false,"given":"S\u00e9rgio Vitorino","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, School of Dentistry Universidade Federal de Uberl\u00e2ndia (UFU). R. Rep\u00fablica Do Piratini  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5134-3466","authenticated-orcid":false,"given":"Maria C\u00e1ssia Ferreira","family":"de Aguiar","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Surgery, School of Dentistry Universidade Federal de Minas Gerais (UFMG). Av. 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