{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:08:17Z","timestamp":1787238497416,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T00:00:00Z","timestamp":1738972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100019446","name":"Medical and Health Science and Technology Development Project of Shandong Province","doi-asserted-by":"publisher","award":["202304030716"],"award-info":[{"award-number":["202304030716"]}],"id":[{"id":"10.13039\/501100019446","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013254","name":"National College Students Innovation and Entrepreneurship Training Program","doi-asserted-by":"publisher","award":["202310439100"],"award-info":[{"award-number":["202310439100"]}],"id":[{"id":"10.13039\/501100013254","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The Rho\u2010associated protein kinase (ROCK) inhibitor Y\u201027632 is a potential immunotherapeutic agent for cancer treatment. Y\u201027632 blocks the growth and migration of oral squamous cell carcinoma (OSCC) CAL\u201027 cells. However, detailed studies on the underlying mechanisms have not yet been reported.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We investigated the effects of Y\u201027632 on the proliferation, migration, and invasion of OSCC cells (CAL\u201027, SCC\u20104, and SCC\u20109) using the Cell Counting Kit\u20108 assay, ethynyl\u20102\u2032\u2010deoxyuridine staining, cell scratch, and transwell assay in\u00a0vitro. Next, ROCK1\/2 was knocked down using siRNA to confirm that the effects of Y\u201027632 were mediated by the inhibition of ROCK activity. A xenograft mouse model was used to verify the effects of Y\u201027632 in\u00a0vivo. The mechanisms underlying Y\u201027632\u2010induced tumor suppression were detected using western blotting and qRT\u2010PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our data demonstrated that Y\u201027632 potently inhibited OSCC cells (CAL\u201027, SCC\u20104, and SCC\u20109) by inhibiting ROCK activity. In\u00a0vivo assays confirmed that Y\u201027632 suppressed OSCC growth by reducing cell proliferation. Biochemical assays demonstrated that Y\u201027632 inactivated the AKT pathway, and treatment with SC79, an AKT activator, rescued the cell growth and migration inhibition elicited by Y\u201027632. Further investigation revealed that Y\u201027632 enhanced autophagy by suppressing the AKT\/mTOR pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study demonstrated that Y\u201027632 significantly suppressed the growth and migration of OSCC cells and upregulated autophagy via the AKT\/mTOR pathway, thus providing a potential therapeutic drug for patients with OSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.13603","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T01:54:16Z","timestamp":1739238856000},"page":"207-216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Y\u201027632 Suppresses the Growth and Migration of Oral Squamous Cell Carcinoma, but Upregulates Autophagy by\u00a0Suppressing\n                    <scp>mTOR<\/scp>\n                    Effectors"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3724-9905","authenticated-orcid":false,"given":"Jie","family":"Wen","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"School of Stomatology Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunhan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"School of Stomatology Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingjian","family":"Zu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"School of Stomatology Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Affiliated Hospital of Heze Medical College  Heze Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"},{"name":"School of Stomatology Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulei","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Stomatology Shandong First Medical University &amp; Shandong Academy of Medical Sciences  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihua","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Periodontology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.29846"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-015-0382-6"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.12203"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2017.01.020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/21541248.2017.1345712"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70378-3"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105093"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20180334"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-35582-1_6"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312916"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-017-3470-7"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088489"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.10.014"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-10-79"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13123026"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-19-0292"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2019.04.011"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3189"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2012.09.010"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2014.3631"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.06.151"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.57761"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-4115-6"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13786"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08996"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-020-00930-2"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073598"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2022.852383"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2022.2063006"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.2526"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21806"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-021-10012-4"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.13603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T04:15:34Z","timestamp":1744344934000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.13603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,8]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/jop.13603"],"URL":"https:\/\/doi.org\/10.1111\/jop.13603","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,8]]},"assertion":[{"value":"2023-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}