{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:17Z","timestamp":1772160737337,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Finance code 001"],"award-info":[{"award-number":["Finance code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Melanocytic neoplasms are rare in the oral cavity and represent a diagnostic challenge due to the overlap between benign and malignant lesions. However, their pathogenesis is not fully elucidated. The aim of this study was to evaluate the expression of the cell cycle\u2010related proteins p16, CDK4, and PTEN in oral melanocytic nevi and melanomas.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A total of 42 cases of melanocytic nevi and five cases of melanoma underwent immunohistochemical analysis. Cases were scored as 0, 1 (<\u20095% of positive cells), 2 (6%\u201350% of positive cells), and 3 (>\u200950% of positive cells). Statistical significance was set at\n p<\/jats:italic>\n \u2009\u2264\u20090.05.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Two cases of melanocytic nevi were totally negative for p16 and 95.2% of cases scored 1. For CDK4, 47.6% of the cases scored 2 and 52.4% scored 3. For PTEN, 97.6% of the cases scored 3 and only one case showed a score of 2. All melanoma cases were classified with a score of 2 for p16, and for CDK4 and PTEN, all cases exhibited a score of 3. PTEN and CDK4 were higher expressed when compared to p16 both in melanocytic nevi and melanomas (\n p<\/jats:italic>\n \u2009<\u20090.001), and melanocytic nevi showed low expression of p16 when compared to melanomas (\n p<\/jats:italic>\n \u2009<\u20090.0001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The findings suggest that these cell cycle\u2010related proteins are not useful biomarkers in melanocytic lesions of the oral mucosa and support the apparent biological distinction between oral and cutaneous lesions.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.13607","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T19:09:29Z","timestamp":1738004969000},"page":"126-129","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Cell Cycle Regulatory Proteins p16,\n CDK4<\/scp>\n , and\n PTEN<\/scp>\n Are Unsuitable as Biomarkers in Oral Melanocytic Lesions"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9564-2318","authenticated-orcid":false,"given":"Aline","family":"Queiroz","sequence":"first","affiliation":[{"name":"Division of Oral and Maxillofacial Pathology, School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2172-9031","authenticated-orcid":false,"given":"Thalita","family":"Santana","sequence":"additional","affiliation":[{"name":"Division of Oral and Maxillofacial Pathology, School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Odontologia School of Dentistry, Ceuma University S\u00e3o Lu\u00eds Maranh\u00e3o Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Fraga Costa","family":"Paris","sequence":"additional","affiliation":[{"name":"Division of Oral and Maxillofacial Pathology, School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8517-2105","authenticated-orcid":false,"given":"Mar\u00edlia","family":"Trierveiler","sequence":"additional","affiliation":[{"name":"Division of Oral and Maxillofacial Pathology, School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14482"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010012\u20100334\u2010y"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12211"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010011\u20100319\u20102"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13180"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017\u20100435\u2010RA"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000428"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3315\/jdcr.2014.1175"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1996.tb07612.x"},{"issue":"8","key":"e_1_2_10_11_1","first-page":"2819","article-title":"Increased CDK4 Protein Expression Predicts a Poor Prognosis in Mucosal Melanoma Associated With the p16INK4a\u2010CDK4\u2010pRb Pathway","volume":"12","author":"Wang F.","year":"2019","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0190\u20109622(03)02473\u20103"}],"container-title":["Journal of Oral Pathology & 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