{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T21:00:53Z","timestamp":1780520453973,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201001318\u201021"],"award-info":[{"award-number":["APQ\u201001318\u201021"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n Salivary glands are the reservoir for severe acute respiratory syndrome Coronavirus 2 virus (SARS\u2010CoV\u20102). It is known that SARS\u2010CoV\u20102 uses angiotensin\u2010converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and FURIN to infect cells. Salivary glands express these entry factors, and it is unclear if these molecules are expressed in salivary glands from primary Sj\u00f6gren's disease (pSjD). This study assessed\n ACE2<\/jats:italic>\n ,\n TMPRSS2<\/jats:italic>\n , and\n FURIN<\/jats:italic>\n gene expression by RT\u2010qPCR in pSjD compared to normal salivary gland and pleomorphic adenoma.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n An integrated study of\n ACE2<\/jats:italic>\n ,\n TMPRSS2<\/jats:italic>\n , and\n FURIN<\/jats:italic>\n gene expression across pSjD, pleomorphic adenoma, and normal salivary gland by RT\u2010pPCR was performed. The association between gene expression and clinicopathological data was also examined.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n pSjD showed ACE2 upregulation compared with normal salivary gland and pleomorphic adenoma (\n p<\/jats:italic>\n \u2009<\u20090.05).\n TMPRSS2<\/jats:italic>\n and\n FURIN<\/jats:italic>\n mRNA levels are also higher in pSjD compared to pleomorphic adenoma (\n p<\/jats:italic>\n \u2009<\u20090.05).\n ACE2<\/jats:italic>\n expression levels were associated with anti\u2010SSA\/SSB positivity (\n p<\/jats:italic>\n \u2009<\u20090.05). A strong correlation was observed between\n TMPRSS2<\/jats:italic>\n and\n FURIN<\/jats:italic>\n in pSjD (\n r<\/jats:italic>\n \u2009=\u20090.768;\n p<\/jats:italic>\n \u2009=\u20090.001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Our analysis revealed a high expression of\n ACE2<\/jats:italic>\n in pSjD.\n ACE2<\/jats:italic>\n ,\n TMPRSS2<\/jats:italic>\n , and\n FURIN<\/jats:italic>\n mRNA levels are increased in pSjD in comparison to pleomorphic adenomas. Neither\n ACE2<\/jats:italic>\n ,\n TMPRSS2<\/jats:italic>\n , nor\n furin<\/jats:italic>\n was consistently associated with SARS\u2010CoV\u20102 positivity in pSjD. These findings may guide future investigations on the effects of SARS\u2010CoV\u20102 in a subset of salivary gland diseases.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.13621","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T03:49:01Z","timestamp":1742183341000},"page":"290-297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["SARS<\/scp>\n \u2010\n CoV<\/scp>\n \u20102 Entry Factors\n \n ACE2<\/i>\n <\/scp>\n ,\n \n TMPRSS2<\/i>\n <\/scp>\n and\n \n FURIN<\/i>\n <\/scp>\n in Salivary Glands From Primary Sj\u00f6gren's Disease"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3054-4642","authenticated-orcid":false,"given":"Fernanda Arag\u00e3o","family":"Felix","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4008-9182","authenticated-orcid":false,"given":"Fl\u00e1via Martins Vasconcelos","family":"Fili\u00fa","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9623-7835","authenticated-orcid":false,"given":"Tarc\u00edlia Aparecida","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5535-3223","authenticated-orcid":false,"given":"D\u00e9bora Cerqueira","family":"Calderaro","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Augusto","family":"Tanure","sequence":"additional","affiliation":[{"name":"Hospital das Cl\u00ednicas da Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6013-9344","authenticated-orcid":false,"given":"Maur\u00edcio Augusto Aquino","family":"de Castro","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8770-8009","authenticated-orcid":false,"given":"Ricardo Santiago","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"},{"name":"Faculdade Ci\u00eancias M\u00e9dicas de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4212-1172","authenticated-orcid":false,"given":"Marina Gon\u00e7alves","family":"Diniz","sequence":"additional","affiliation":[{"name":"Department of Pathology Biological Science Institute, Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7820-4749","authenticated-orcid":false,"given":"S\u00edlvia Ferreira","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010022\u201000711\u20102"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010021\u201000418\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.594495"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13560"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath\u20102020\u2010206788"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.726753"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510396882"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591\u2010021\u201001296\u20108"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14752"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39859"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keaa898"},{"issue":"3","key":"e_1_2_11_13_1","first-page":"47","article-title":"Proprotein 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