{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:18Z","timestamp":1772160738860,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071129"],"award-info":[{"award-number":["82071129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301102"],"award-info":[{"award-number":["82301102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Fibrosis is a disease characterized by excessive collagen deposition by fibroblasts, leading to tissue and organ dysfunction. Fibroblasts are the primary effector cells, and their functional phenotype is regulated by various factors, with metabolic reprogramming being a crucial one. Previous studies have shown that microRNAs induce hypoxia, abnormal energy metabolism, and promote fibrosis. Our research aimed to identify the miRNA associated with oral submucous fibrosis (OSF) pathogenesis and to preliminarily explore its role in the phenotypic transformation of fibroblasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      RNA\u2010seq was used to analyze the differential expression of miRNA in OSF and normal tissues, followed by validation in clinical samples (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914), saliva (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200965), and animal models (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200916). The effect of hsa\u2010miR\u2010134\u20103p on collagen synthesis was detected by qPCR. Bioinformatic, immunoblot, and Elisa methods were used to analyze the correlation between hsa\u2010miR\u2010134\u20103p and methionine metabolism, as well as the mTOR pathway. Finally, the luciferase reporter gene assay was used to identify the target gene of hsa\u2010miR\u2010134\u20103p.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Hsa\u2010miR\u2010134\u20103p is underexpressed in fibrotic tissues. Arecoline can promote collagen synthesis by reducing hsa\u2010miR\u2010134\u20103p. Bioinformatic analysis indicated that hsa\u2010miR\u2010134\u20103p is associated with methionine metabolism and mTOR signaling, and immunoblot and ELISA methods revealed that hsa\u2010miR\u2010134\u20103p regulates methionine metabolism via the mTOR pathway. Ultimately, hsa\u2010miR\u2010134\u20103p was identified to directly target SLC25A33 in fibroblasts using the luciferase reporter gene assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In summary, the findings suggested that miR\u2010134\u20103p is underexpressed in fibroblasts, which directly binds to SLC25A33, leading to degradation of SLC25A33 mRNAs. SLC25A33 induces methionine metabolism reprogramming to promote collagen synthesis through the mTOR pathway in OSF.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70003","type":"journal-article","created":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T06:24:56Z","timestamp":1751091896000},"page":"584-596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>miR<\/scp>\n                    \u2010134\u20103p Deficiency\u2010Induced Methionine Metabolism Promotes Fibrosis via\n                    <scp>SLC25A33<\/scp>\n                    in\n                    <scp>OSF<\/scp>"],"prefix":"10.1111","volume":"54","author":[{"given":"Mian\u2010Feng","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Endodontics, School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction &amp; Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine Guangzhou Medical University  Guangzhou People's Republic of China"},{"name":"Department of Stomatology, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Institute of Oral Precancerous Lesions Central South University  Changsha People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng\u2010Ying","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Changsha Stomatological Hospital, School of Stomatology Hunan University of Chinese Medicine  Changsha People's Republic of 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Stomatology, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Institute of Oral Precancerous Lesions Central South University  Changsha People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Le","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital Central South University  Changsha People's Republic of China"},{"name":"Institute of Oral Precancerous Lesions Central South University  Changsha People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of 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