{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:20Z","timestamp":1772160740246,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T00:00:00Z","timestamp":1754697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["407364\/2021\u20108"],"award-info":[{"award-number":["407364\/2021\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312830\/2022\u20100"],"award-info":[{"award-number":["312830\/2022\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Odontogenic keratocyst (OKC) treatment often involves enucleation followed by peripheral ostectomy to reduce recurrence. However, identifying bone areas containing epithelial remnants remains a surgical challenge. The intraoperative use of 1% methylene blue solution has been proposed to delineate residual epithelial tissue. This study aimed to evaluate the presence of epithelial structures in both OKC surgical specimens and bone tissue stained with 1% methylene blue solution following enucleation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A retrospective analysis was conducted on histological records from OKC cases treated between August 2022 and December 2023. The primary variable was the presence of epithelial structures, including daughter cysts, microcysts, and epithelial remnants. Data were analyzed descriptively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Twelve patients (mean age: 40.8\u2009years) were included. Most lesions were located in the posterior mandible (50%) and exhibited a unilocular radiographic pattern (91.7%), with a mean lesion size of 25\u2009mm. Epithelial structures were identified in 75% of OKC surgical specimens and in 16.7% of the stained bone tissues. The mean follow\u2010up period was 26.5\u2009months, with no recurrences observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The use of 1% methylene blue solution may aid in the intraoperative identification of residual epithelial tissue, potentially contributing to more precise and conservative OKC management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70031","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T10:05:34Z","timestamp":1754733934000},"page":"770-776","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of Tissue Staining With 1% Methylene Blue Solution After Enucleation of Odontogenic Keratocyst"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4215-7922","authenticated-orcid":false,"given":"Gustavo Henrique","family":"Martins","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry School of Dentistry, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Alcides Almeida","family":"de Arruda","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis and Pathology School of Dentistry, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Augusto Benevenuto","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis and Pathology School of Dentistry, Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayssa Nunes","family":"Villafort","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Complexo Hospitalar S\u00e3o Francisco  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio Bruno Figueiredo","family":"do Amaral","sequence":"additional","affiliation":[{"name":"Residency Program of Oral and Maxillofacial Surgery Hospital Jo\u00e3o XXIII\/FHEMIG  Belo Horizonte Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6657-4547","authenticated-orcid":false,"given":"Felipe Paiva","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry School of Dentistry, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2258-8071","authenticated-orcid":false,"given":"Lucas Guimar\u00e3es","family":"Abreu","sequence":"additional","affiliation":[{"name":"Department of Child and Adolescent Oral Health School of Dentistry, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3207-4007","authenticated-orcid":false,"given":"Ricardo Alves","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pathology and Clinical Dentistry School of Dentistry, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12663-014-0734-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2015.03.008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24400"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2021.02.015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2023.04.006"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12663-023-01929-0"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-020-00866-4"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11282-020-00425-2"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2016.01.015"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-024-01854-z"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.5905"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2022.09.004"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2011.02.008"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12663-022-01846-8"},{"issue":"4","key":"e_1_2_10_16_1","first-page":"e491","article-title":"Recurrence of Odontogenic Keratocysts and Possible Prognostic Factors: Review of 455 Patients","volume":"24","author":"Fidele N. B.","year":"2019","journal-title":"Medicina Oral, Patolog\u00eda Oral y Cirug\u00eda Bucal"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12031133"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-023-05313-7"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13278"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2012.09.006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10006-021-00994-4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00729.x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2016.11.010"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2001.113109"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200008000-00020"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(72)90366-0"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(77)90161-X"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.70031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:41:26Z","timestamp":1757464886000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.70031"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,9]]},"references-count":26,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/jop.70031"],"URL":"https:\/\/doi.org\/10.1111\/jop.70031","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JOP.70031\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70031\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70031\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70031\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70031\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70031\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70031\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,9]]},"assertion":[{"value":"2024-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}