{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:52:25Z","timestamp":1772160745503,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T00:00:00Z","timestamp":1755129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004482","name":"Kuwait University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004482","name":"Kuwait University","doi-asserted-by":"publisher","award":["SRUL 01\/14"],"award-info":[{"award-number":["SRUL 01\/14"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n \n To investigate the interaction between fluconazole\u2010resistant (Flu\u2010R) and \u2010susceptible dose\u2010dependent (Flu\u2010SDD) isolates of\n \n Candida albicans<\/jats:italic>\n <\/jats:styled-content>\n and\n Candida glabrata<\/jats:italic>\n with oral\n streptococci<\/jats:italic>\n , exploring autoaggregation, coaggregation, and the impact of streptococcal biofilm\u2010secreted components on\n Candida<\/jats:italic>\n biofilms.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Autoaggregation and coaggregation of\n Candida<\/jats:italic>\n Flu\u2010R and Flu\u2010SDD isolates with\n streptococci<\/jats:italic>\n (\n \n S. mutans<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n S. gordonii<\/jats:italic>\n <\/jats:styled-content>\n , and\n \n S. sanguinis<\/jats:italic>\n <\/jats:styled-content>\n ) were assessed using an optical density assay. The inhibitory effects of streptococcal biofilm\u2010secreted components on\n Candida<\/jats:italic>\n biofilms were examined, quantifying biofilm inhibition by crystal violet staining and assessing viability through 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assay. Statistical analysis of the data was done by one\u2010way ANOVA, considering a\n p<\/jats:italic>\n \u2010value of <\u20090.05 as significant.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Flu\u2010R\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n exhibited higher autoaggregation (71%) than Flu\u2010SDD (62%), both surpassing\n Streptococcus<\/jats:italic>\n spp. (32%\u201349%). Flu\u2010R and Flu\u2010SDD\n \n C. glabrata<\/jats:italic>\n <\/jats:styled-content>\n had less autoaggregation ability than\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n (\n p<\/jats:italic>\n \u2009<\u20090.05). Coaggregation increased steadily, with Flu\u2010SDD\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n exhibiting the highest coaggregation with\n \n S. mutans<\/jats:italic>\n <\/jats:styled-content>\n (69%\u2009\u00b1\u20098% at 2\u2009h). Flu\u2010R strains showed significant coaggregation differences with streptococcal species (\n p<\/jats:italic>\n \u2010values 0.05\u2013<\u20090.001). Biofilm inhibition was significant in\n Candida<\/jats:italic>\n Flu\u2010R and Flu\u2010SDD isolates treated with streptococcal biofilm supernatants. Supernatants of all three streptococcal species decreased Flu\u2010R\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n viability (1.15\u20132.15\u2010fold).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Fluconazole susceptibility\/resistance significantly influences aggregation and biofilm formation with oral\n streptococci<\/jats:italic>\n . Streptococcal biofilm supernatants hinder\n Candida<\/jats:italic>\n strains' growth and viability, suggesting implications for colonization, biofilm formation, and oral infections.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.70034","type":"journal-article","created":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T01:39:11Z","timestamp":1755221951000},"page":"853-862","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Influence of Fluconazole Resistance and Susceptibility on\n Candida<\/i>\n \u2013Streptococci Aggregation Dynamics"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8927-6617","authenticated-orcid":false,"given":"Maribasappa","family":"Karched","sequence":"first","affiliation":[{"name":"Oral Microbiology Research Laboratory, Department of Bioclinical Sciences College of Dentistry, Kuwait University Kuwait 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