{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:31:40Z","timestamp":1781623900357,"version":"3.54.5"},"reference-count":10,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["FRGS\/1\/2022\/SKK11\/UIAM\/02\/2"],"award-info":[{"award-number":["FRGS\/1\/2022\/SKK11\/UIAM\/02\/2"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n Candida<\/jats:italic>\n species and\n \n Staphylococcus aureus<\/jats:italic>\n <\/jats:styled-content>\n are nosocomial pathogens associated with immunocompromised individuals, especially oral cancer patients. This study elucidates the effects of mono\u2010 and co\u2010culture biofilms of\n \n Candida albicans<\/jats:italic>\n <\/jats:styled-content>\n ,\n Candida auris<\/jats:italic>\n and\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n on cell viability and pro\u2010inflammatory cytokine expression in healthy epithelial cells (hTERT TIGKs) and oral cancer (ORL\u201048) cell lines.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Mono\u2010 and co\u2010culture biofilms of\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n C. auris<\/jats:italic>\n <\/jats:styled-content>\n and\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n , developed using static biofilm for 72\u2009h, were collected and filter sterilized (biofilm filtrate). Test cell growth medium (TCGM) was prepared for hTERT TIGKs and ORL\u201048 by mixing 20% (v\/v) biofilm filtrate with 80% serum\u2010free media. The cells were seeded in 96\u2010well plates, and TCGM was added as treatment. The unstimulated media (UM), made of 100% serum\u2010free media, served as control. After 24\u2009h, cell viability was assessed using CCK\u20108. Interleukin (IL)\u20106 and IL\u20108 expression was analysed using enzyme\u2010linked immunosorbent assay (ELISA).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n consistently decreased cell vitality in co\u2010culture as compared to mono\u2010culture,\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n and\n \n C. auris<\/jats:italic>\n <\/jats:styled-content>\n in both cell lines. Co\u2010culture of\n \n C. auris<\/jats:italic>\n <\/jats:styled-content>\n and\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n in cancer cells demonstrated significantly higher IL\u20106 and IL\u20108 expression than\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n (\n p<\/jats:italic>\n \u2009<\u20090.05). Furthermore, co\u2010culturing\n \n C. auris<\/jats:italic>\n <\/jats:styled-content>\n or\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n with\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n increases pro\u2010inflammatory IL\u20108 expression in oral cancer cell lines compared to mono\u2010cultures of respective\n Candida<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Co\u2010culturing\n \n C. auris<\/jats:italic>\n <\/jats:styled-content>\n or\n \n C. albicans<\/jats:italic>\n <\/jats:styled-content>\n with\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n reduced cell viability and increased pro\u2010inflammatory IL\u20108 expression in oral cancer cell lines, suggesting that interkingdom interaction may regulate oral carcinogenesis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.70043","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T03:21:08Z","timestamp":1755746468000},"page":"909-915","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Mono\u2010 and Co\u2010Culture Biofilms of\n Candida auris<\/i>\n or\n Candida albicans<\/i>\n With\n Staphylococcus aureus<\/i>\n Regulate Cell Viability and Pro\u2010Inflammatory Cytokine Expression Differently in Oral Cancer Cell Lines"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2393-0425","authenticated-orcid":false,"given":"Wan NurHazirah","family":"Wan Ahmad Kamil","sequence":"first","affiliation":[{"name":"Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry International Islamic University Malaysia Kuantan Pahang Malaysia"},{"name":"Cluster of Cancer Research Initiative IIUM (COCRII) International Islamic University Malaysia Kuantan Pahang Malaysia"},{"name":"Centre of Oral Maxillofacial Diagnostic Medicine and Studies, Faculty of Dentistry Universiti Teknologi MARA (UiTM) Sungai Buloh Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munirah","family":"Mokhtar","sequence":"additional","affiliation":[{"name":"Department of Medical Diagnostic, Faculty of Health Sciences Universiti Selangor Shah Alam Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madia Baizura","family":"Baharom","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Kulliyyah of Medicine International Islamic University Malaysia Kuantan Pahang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. M. H. N.","family":"Bandara","sequence":"additional","affiliation":[{"name":"Bristol Dental School University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2512-1557","authenticated-orcid":false,"given":"Mohammad S.","family":"Alrashdan","sequence":"additional","affiliation":[{"name":"Department of Oral and Craniofacial Health Sciences, College of Dental Medicine University of Sharjah Sharjah UAE"},{"name":"Department of Oral Medicine and Oral Surgery, Faculty of Dentistry Jordan University of Science and Technology Irbid Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1429-1323","authenticated-orcid":false,"given":"Nicola","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Melbourne Dental School The University of Melbourne Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9470-6412","authenticated-orcid":false,"given":"Mohd Hafiz","family":"Arzmi","sequence":"additional","affiliation":[{"name":"Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry International Islamic University Malaysia Kuantan Pahang Malaysia"},{"name":"Cluster of Cancer Research Initiative IIUM (COCRII) International Islamic University Malaysia Kuantan Pahang Malaysia"},{"name":"Melbourne Dental School The University of Melbourne Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368\u2010023\u201000249\u2010w"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2023.127370"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.JOMFP_20_20"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed9010013"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25010395"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2025.2518281"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903\u2010024\u201004840\u20103"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgy137"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008921"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb44090274"}],"container-title":["Journal of Oral Pathology & Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.70043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T02:46:08Z","timestamp":1760496368000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.70043"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":10,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/jop.70043"],"URL":"https:\/\/doi.org\/10.1111\/jop.70043","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JOP.70043\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70043\/v2\/decision1","asserted-by":"object"}]},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,20]]},"assertion":[{"value":"2025-01-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}