{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:55:42Z","timestamp":1782921342457,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["Project number: 1919B012303331"],"award-info":[{"award-number":["Project number: 1919B012303331"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Despite its widespread use in acne vulgaris treatment, the potential cytological and genotoxic effects of systemic isotretinoin on oral mucosal cells remain poorly understood. This study aimed to investigate the cytological and genotoxic effects of systemic isotretinoin on oral mucosal cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Buccal smear samples were collected from isotretinoin users (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920) and controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920). Cytomorphometric analyses (cell diameter (CD), nuclear diameter (ND), nuclear area (NA), cell area (CA), ND\/CD, and NA\/CA ratios) and cytological evaluations (nuclear degenerations, binucleation, perinuclear halos) were conducted using Papanicolaou staining. Micronucleus (MN) testing was performed on Feulgen\u2010stained samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CD, ND, CA, and NA were significantly higher in the isotretinoin group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), while the ND\/CD ratio was reduced (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). MN scores and the presence of micronucleated cells (MNed cells) were significantly increased in isotretinoin users (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), indicating genotoxic effects. The isotretinoin group showed a lower mean pH (5.8) than controls (6.7) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). No significant differences were linked to isotretinoin duration (less than 6\u2009months (6&lt;) vs. 6\u2009months or more (6\u2265)) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Isotretinoin use induces cytological changes, including increased cell and nuclear sizes, and genotoxic effects, as evidenced by elevated MN formation. Monitoring oral mucosal alterations in isotretinoin users may provide insights into potential side effects.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70048","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T04:54:54Z","timestamp":1755924894000},"page":"944-952","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cytological and Genotoxic Effects of Systemic Isotretinoin on Oral Mucosa Cells"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9964-4057","authenticated-orcid":false,"given":"Alinda","family":"Uslu","sequence":"first","affiliation":[{"name":"Department of Biology Hacettepe University, Faculty of Science  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7413-4939","authenticated-orcid":false,"given":"Hanife Guler","family":"Donmez","sequence":"additional","affiliation":[{"name":"Department of Biology Hacettepe University, Faculty of Science  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2019.08.014"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1610\u20100387.2009.07238.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.14438"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2009.03.005"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/derm.1.3.9364"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14798"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.55946"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22157821"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2018.08.010"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467215666220520143124"},{"issue":"8","key":"e_1_2_10_12_1","first-page":"e124","article-title":"Effect of Systemic Isotretinoin Therapy on Mucociliary Clearance and Nasal Surface Mucosa in Acne Patients","volume":"12","author":"Takci Z.","year":"2013","journal-title":"Journal of Drugs in Dermatology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/09546634.2012.697985"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23803"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000518582"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976\u20109668.166055"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijpm.ijpm_43_22"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100714.2003.00162.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100041\u20101740348"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.4662"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.53"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201025311\u20100"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1383\u20105742(99)00053\u20108"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973\u20101482.98968"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105\u2010022\u201001413\u20100"},{"issue":"4","key":"e_1_2_10_26_1","first-page":"1955","article-title":"Cytogenetic Biomonitoring in Buccal Mucosa Cells From Women Submitted to Chemotherapy After Mastectomy for Breast Cancer","volume":"36","author":"Souza A. 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