{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:27:04Z","timestamp":1790314024217,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["409081\u201021\u20103"],"award-info":[{"award-number":["409081\u201021\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["151496\/2022\u20106"],"award-info":[{"award-number":["151496\/2022\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307318\/2022\u20102"],"award-info":[{"award-number":["307318\/2022\u20102"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u20103020\/21"],"award-info":[{"award-number":["APQ\u20103020\/21"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Oral squamous cell carcinoma (OSCC) is one of the most frequent head and neck cancers. The 4\u2010nitroquinoline 1\u2010oxide (4NQO) mouse model of oral carcinogenesis is a well\u2010established model to investigate the mechanism behind OSCC development, including epigenetic alterations. Studies have shown that histone acetylation is a key regulator of gene expression and may play a role in such a tumor. This study investigates the acetylation of H3K9, H3K14, and H3K27 and the\n                      <jats:italic>KAT2A<\/jats:italic>\n                      gene expression in a sex\u2010based approach in a 4NQO\/ethanol (EtOH)\u2010induced OSCC mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 120 C57Bl\/6J mice (60 males and 60 females) were divided into four groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200915). In the first 10\u2009weeks, they were treated with 5\u2009mg\/mL propylene glycol (PPG) or 100\u2009\u03bcg\/mL 4NQO in drinking water, followed by either sterilized water or 8% EtOH for 15\u2009weeks. After euthanasia, tongues were analyzed histopathologically. Immunohistochemistry and qPCR were also employed to study the histones and gene expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Female mice showed increased H3K9ac and H3K14ac expression from normal mucosa to dysplasia, followed by decreased expression in OSCC. H3K9ac and H3K14ac expression in males was lower in the 4NQO\/EtOH group. H3K27ac was higher in dysplastic lesions compared to OSCC, particularly in females. Comparatively, females had higher H3K9ac and H3K14ac expression in the 4NQO\/EtOH group than males.\n                      <jats:italic>KAT2A<\/jats:italic>\n                      expression was lower in females treated with PPG\/EtOH and 4NQO\/H\n                      <jats:sub>2<\/jats:sub>\n                      O than in males.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our results indicate that H3 acetylation and\n                      <jats:italic>KAT2A<\/jats:italic>\n                      gene expression may play a key role in oral carcinogenesis on a sex\u2010related basis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70062","type":"journal-article","created":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T11:30:01Z","timestamp":1757503801000},"page":"1074-1084","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sex\u2010Related Differences in Histone Acetylation and Tumor Development in a 4\u2010Nitroquinoline 1\u2010Oxide and Ethanol\u2010Induced Oral Squamous Cell Carcinoma Mouse Model"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2297-1882","authenticated-orcid":false,"given":"Wender Rodrigues","family":"Naz\u00e1rio","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7560-6531","authenticated-orcid":false,"given":"Ana\u00edra Ribeiro Guedes 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