{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T13:04:17Z","timestamp":1789736657280,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["5\/4\/2\u20103\/OH\/2022\u2010NCD\u2010II"],"award-info":[{"award-number":["5\/4\/2\u20103\/OH\/2022\u2010NCD\u2010II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Oral submucous fibrosis is a pre\u2010malignant disorder caused by habitual areca nut consumption. This pilot study investigated the therapeutic potential of dental pulp mesenchymal stem cell\u2010secretome chitosan gel in a mice model of areca nut extract\u2010induced oral submucous fibrosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Angiogenic potential of dental pulp mesenchymal stem cell\u2010secretome was validated using a chick yolk sac membrane assay. Oral submucous fibrosis was induced in male Swiss albino mice (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200940) via intraoral areca nut extract administration for 3\u2009months. Post\u2010induction, animals were divided into disease control, DPMSCs\u2010S, and DPMSCs\u2010S gel groups. Treatments were administered intraorally twice weekly for 1\u2009month. Therapeutic efficacy was assessed through measurements of mouth opening, histopathology, oxidative stress markers (LDH, MDA, SOD), and fibrotic gene expression (COL1, COL3, \u03b1\u2010SMA).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Dental pulp mesenchymal stem cells\u2019 secretome contains pro\u2010angiogenic growth factors. Dental pulp mesenchymal stem cells\u2019 secretome gel significantly improved mouth opening, restored epithelial architecture, and reduced collagen deposition. Histological staining and gene expression analyses confirmed the reversal of fibrosis and downregulation of COL1, COL3, and \u03b1\u2010SMA. Additionally, the gel reduced LDH and MDA levels and enhanced SOD activity, indicating antioxidant effects. The gel showed superior efficacy over the secretome alone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>DPMSCs\u2010S gel demonstrates significant anti\u2010fibrotic, antioxidant, and regenerative potential in reversing ANE\u2010induced OSMF in mice. These findings warrant further investigation into larger, long\u2010term preclinical studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70064","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T10:08:40Z","timestamp":1758190120000},"page":"161-167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dental Pulp Mesenchymal Stem Cell\u2010Secretome Gel Reverse Areca Nut Induced Oral Submucous Fibrosis in Mice: A Pilot Study"],"prefix":"10.1111","volume":"55","author":[{"given":"Nishant","family":"Mante","sequence":"first","affiliation":[{"name":"Regenerative Medicine Laboratory Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri  Pune India"},{"name":"Department of Pharmacology, School of Pharmacy and Research Dr. D. Y. Patil Dnyan Prasad University  Pimpri, Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supriya","family":"Kheur","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri  Pune India"},{"name":"Department of Oral Maxillofacial Pathology Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash","family":"Sanap","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5647-8340","authenticated-orcid":false,"given":"Avinash","family":"Kharat","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranjali","family":"Potdar","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonam","family":"Suryawanshi","sequence":"additional","affiliation":[{"name":"Central Research Facility Dr. D. Y. 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Patil Institute of Pharmaceutical Sciences and Research  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitika","family":"Monga","sequence":"additional","affiliation":[{"name":"Division of Developmental Research Indian Council of Medical Research Headquarters  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh","family":"Bhonde","sequence":"additional","affiliation":[{"name":"Regenerative Medicine Laboratory Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/njms.NJMS_32_17"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ors.12771"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9967097"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6631856"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222112018"},{"issue":"4","key":"e_1_2_10_7_1","first-page":"323","article-title":"Assessment of Angiogenic Potential of Mesenchymal Stem Cells Derived Conditioned Medium From Various Oral Sources","volume":"8","author":"Shekatkar M. 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