{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T06:12:58Z","timestamp":1771222378138,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970536"],"award-info":[{"award-number":["81970536"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Elucidating the immune cell network is of great significance for understanding the pathogenesis of periodontitis. Flow cytometry is the preferred tool for quantifying the composition of immune cells in the pathological process of inflammation. Perfusion prior to tissue harvesting is necessary for flow cytometric analysis of various tissues. However, whether perfusion is necessary for flow cytometric analysis of murine gingival tissues has never been reported.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Forty\u2010eight mice were randomly divided into four groups: control\u2009+\u2009non\u2010perfused group, control\u2009+\u2009perfused group, periodontitis\u2009+\u2009non\u2010perfused group, and periodontitis\u2009+\u2009perfused group. Ligation\u2010induced experimental periodontitis was established in periodontitis mice. The circulating blood was perfused out in the perfused groups before harvesting tissue samples. Flow cytometry was used to analyze the composition of immune cells in murine palatal gingival tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Experimental periodontitis induced alveolar bone resorption and inflammatory cell infiltration. Perfusion can successfully remove blood from the vessels of murine periodontal tissues. Flow cytometric analysis showed that there was no significant difference in the proportion of various immune cells in murine gingival tissues between the perfusion groups and the non\u2010perfusion groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present study was the first to use flow cytometry to compare perfused mouse gingiva with non\u2010perfused mouse gingiva and found no significant difference in immune cell composition between the two groups. In addition, this study provides a simple and efficient protocol for the collection and processing of murine gingival tissue, which could provide facilities for understanding the role and mechanism of the immune cell network in periodontal diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70074","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T05:00:55Z","timestamp":1761714055000},"page":"191-200","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Simple and Efficient Method for Analyzing Murine Gingival Immune Cells Using Flow Cytometry: An Animal Study"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2491-5569","authenticated-orcid":false,"given":"Wenting","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Periodontology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology Peking University School and Hospital of Stomatology  Beijing China"},{"name":"Fujian Key Laboratory of Oral Diseases &amp; Fujian Provincial Engineering Research Center of Oral Biomaterial &amp; Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology Fujian Medical University  Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuekang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, and NHC Key Laboratory of Medical Immunology Peking University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiping","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Periodontology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, 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