{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T03:19:25Z","timestamp":1784085565321,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2020\/10362\u20100"],"award-info":[{"award-number":["#2020\/10362\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2020\/10322\u20109"],"award-info":[{"award-number":["#2020\/10322\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The packaging and marketing of electronic cigarettes (e\u2010cigs) often target younger demographics. This study aimed to evaluate gene expression in e\u2010cig users through exfoliative cytology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Samples were collected from 17 e\u2010cig users and 10 nonsmokers as controls. Clinical data included age, gender, heart rate, oximetry, capillary blood glucose, carbon monoxide levels, sialometry, alcohol\u2010related risk scores, alcohol consumption, and e\u2010cig use parameters. Smears from the left tongue edge were obtained using a Rovers Orcellex Brush. The Papanicolaou method assessed epithelial maturation and cytological features, categorized from normal to conclusive for malignancy. Cellular composition, inflammation, microbial presence, and atypia were evaluated using a semiquantitative scoring system. Gene expression (p16, IL1\u2010beta, CXCL8, TNF, and KRT13) was analyzed by RT\u2010PCR. Statistical comparisons used the Mann\u2013Whitney test, and correlations were assessed via Spearman's test (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Fruit flavors were the most preferred. Some users were former smokers (average abstention: 3.15\u2009months). Bacterial colonies were more prevalent in the e\u2010cig group (64.7% vs. 20%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.085), mucus and inflammatory changes were found exclusively in e\u2010cig users (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.062). No significant differences were found in the Papanicolaou classification by gender (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.904). Gene expression analysis showed a differential expression of p16 and TNF between the groups. Significant correlations were found between carbon monoxide and p16 expression (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.41,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02), vaping sessions per day and p16 expression (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.37,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02), and daily alcohol dose and TNF expression (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.42,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.04).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>E\u2010cigarette use may induce early molecular and cytological changes in the oral mucosa, affecting inflammation, immunity, and epithelial differentiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70095","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T06:07:09Z","timestamp":1764310029000},"page":"331-339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of Electronic Nicotine Delivery Systems on Oral Mucosa: A Cytopathological and Molecular Study"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5174-8922","authenticated-orcid":false,"given":"Alba","family":"P\u00e9rez\u2010Jard\u00f3n","sequence":"first","affiliation":[{"name":"Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry University of Santiago de Compostela  Santiago de Compostela Spain"},{"name":"ORALRES Group, Health Research Institute of Santiago de Compostela (FIDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2064-5220","authenticated-orcid":false,"given":"Bruna Fernandes do Carmo","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP), Instituto de Ci\u00eancia e Tecnologia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5637-9908","authenticated-orcid":false,"given":"Cintia Micaela","family":"Chamorro\u2010Petronacci","sequence":"additional","affiliation":[{"name":"Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry University of Santiago de Compostela  Santiago de Compostela Spain"},{"name":"ORALRES Group, Health Research Institute of Santiago de Compostela (FIDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5792-8350","authenticated-orcid":false,"given":"Maria Dolores","family":"Reboiras\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry University of Santiago de Compostela  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1769-1755","authenticated-orcid":false,"given":"Natalia de Carvalho","family":"Faria","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP), Instituto de Ci\u00eancia e Tecnologia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5970-0464","authenticated-orcid":false,"given":"Renata Falchete do","family":"Prado","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP), Instituto de Ci\u00eancia e Tecnologia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3411-9443","authenticated-orcid":false,"given":"M\u00f3nica Ghislaine Oliveira","family":"Alves","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP), Instituto de Ci\u00eancia e Tecnologia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8389-086X","authenticated-orcid":false,"given":"F\u00e1bio Fran\u00e7a Vieira e","family":"Silva","sequence":"additional","affiliation":[{"name":"Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry University of Santiago de Compostela  Santiago de Compostela Spain"},{"name":"ORALRES Group, Health Research Institute of Santiago de Compostela (FIDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9302-7768","authenticated-orcid":false,"given":"Maria\u2010Elena","family":"Pad\u00edn\u2010Iruegas","sequence":"additional","affiliation":[{"name":"Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry University of Santiago de Compostela  Santiago de Compostela Spain"},{"name":"ORALRES Group, Health Research Institute of Santiago de Compostela (FIDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-9868","authenticated-orcid":false,"given":"Mario","family":"P\u00e9rez\u2010Say\u00e1ns","sequence":"additional","affiliation":[{"name":"Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes), Faculty of Medicine and Dentistry University of Santiago de Compostela  Santiago de Compostela Spain"},{"name":"ORALRES Group, Health Research Institute of Santiago de Compostela (FIDIS)  Santiago de Compostela Spain"},{"name":"Instituto de los Materiales de Santiago de Compostela (iMATUS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4596-9715","authenticated-orcid":false,"given":"Janete Dias","family":"Almeida","sequence":"additional","affiliation":[{"name":"Universidade Estadual Paulista (UNESP), Instituto de Ci\u00eancia e Tecnologia  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/09603271221101052"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina60030365"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.puhe.2023.03.017"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2020.11.009"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030738"},{"issue":"1","key":"e_1_2_13_7_1","first-page":"9","article-title":"Comparative Study of Oral Mucosa Micronuclei in Smokers and Alcoholic Smokers","volume":"34","author":"Oliveira L. U.","year":"2012","journal-title":"Analytical and Quantitative Cytology and Histology"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2016.09.006"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.02.039"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000133"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2023.154656"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM\u2010190776"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo11100650"},{"issue":"3","key":"e_1_2_13_14_1","first-page":"211","article-title":"The Diagnostic Value of Vaginal Smears in Carcinoma of the Uterus","volume":"121","author":"Papanicolaou G. N.","year":"1941","journal-title":"Archives of Pathology & Laboratory Medicine"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2024.03.009"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2017.10.001"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addbeh.2021.107220"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addbeh.2013.10.028"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194145"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252111750"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201906\u2010472OC"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.18001\/TRS.4.5.4"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ntr\/nts103"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/add.13183"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117222"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12971\u2010014\u20100023\u20106"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12810"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2023.1146949"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41432\u2010023\u201000929\u2010w"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325988"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41432\u2010023\u201000956\u20107"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.07.003"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177780"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.314"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12030448"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826084.2021.1887256"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.70095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jop.70095","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.70095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:44:24Z","timestamp":1773002664000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.70095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/jop.70095"],"URL":"https:\/\/doi.org\/10.1111\/jop.70095","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/JOP.70095\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70095\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/JOP.70095\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]},"assertion":[{"value":"2025-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}