{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:15:36Z","timestamp":1778127336745,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201000703\u201022"],"award-info":[{"award-number":["APQ\u201000703\u201022"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Odontogenic keratocyst (OKC) is a developmental odontogenic cyst characterized by a fragile epithelial lining attachment to the capsula and high recurrence rates. Matrix metalloproteinases (MMPs) and adhesion\u2010related proteins (TLN1, FBLN1) may contribute to epithelial detachment, which could facilitate lesion recurrence. This study investigated the mRNA expression of\n                      <jats:italic>MMP2<\/jats:italic>\n                      ,\n                      <jats:italic>MMP9, TLN1<\/jats:italic>\n                      , and\n                      <jats:italic>FBLN1<\/jats:italic>\n                      in OKCs and their association with epithelial detachment, including the impact of marsupialization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Gene expression was analyzed by RT\u2010qPCR in samples from OKCs (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910), marsupialized OKCs (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095), and oral mucosa controls (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094). Gelatinase activity was assessed by gelatin zymography in fresh samples (OKCs\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097; oral mucosa\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094). Histological sections were digitally measured to quantify areas of epithelial detachment. Correlation analyses were performed between mRNA expression levels and epithelial separation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>MMP2<\/jats:italic>\n                      and\n                      <jats:italic>MMP9<\/jats:italic>\n                      mRNA levels were significantly higher in OKCs compared to oral mucosa (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), with decreased mRNA levels after marsupialization. Gelatin zymography confirmed elevated gelatinase activity, particularly for MMP9, in OKC tissues.\n                      <jats:italic>TLN1<\/jats:italic>\n                      and\n                      <jats:italic>FBLN1<\/jats:italic>\n                      mRNA expressions were also elevated in OKCs, consistent with previous proteomic data, though not statistically significant. A significant correlation was observed between\n                      <jats:italic>MMP9<\/jats:italic>\n                      mRNA expression and the total area of detached epithelium.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>MMP9<\/jats:italic>\n                      may play a critical role in epithelial detachment in OKCs and could serve as a therapeutic target or prognostic marker. Marsupialization appears to modulate matrix metalloproteinases expression, suggesting a molecular mechanism for its clinical benefits.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70112","type":"journal-article","created":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T06:21:28Z","timestamp":1767421288000},"page":"532-538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Insights Into Epithelial Detachment in Odontogenic Keratocyst: The Role of Matrix Metalloproteinases and Effects of Marsupialization"],"prefix":"10.1111","volume":"55","author":[{"given":"Arthur Henrique Soares","family":"Pacheco","sequence":"first","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Rocha Fonseca","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology, School of Dentistry Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alline Teixeira","family":"Valeriano","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savio Almeida","family":"Botrel","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"La\u00eds Santos","family":"C\u00e2mara","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Gabrielle","family":"De Sousa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Cavalieri","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa de F\u00e1tima","family":"Bernardes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Santiago","family":"Gomez","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology, School of Dentistry Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"},{"name":"Faculty of Medical Sciences of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Gon\u00e7alves","family":"Diniz","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais (UFMG)  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"WHO Classification of Head and Neck 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