{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T01:46:26Z","timestamp":1785721586275,"version":"3.56.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K23635"],"award-info":[{"award-number":["24K23635"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K20255"],"award-info":[{"award-number":["25K20255"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Perineural invasion (PNI) is a pathological feature of oral squamous cell carcinoma (OSCC) that is associated with a poor prognosis. However, the molecular mechanisms remain poorly understood. The aim of this study was to establish an in\u00a0vivo model to explore PNI in OSCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed an integrated analysis of The Cancer Genome Atlas transcriptome data from tongue squamous cell carcinoma (TSCC) and floor of the mouth squamous cell carcinoma (FOMSCC) by the presence of PNI and identified differentially expressed genes (DEGs). We also performed xenografts of five OSCC cell lines (OSC19, OSC20, HSC2, KOSC2, and HO\u20101\u2010u\u20101) into nude mouse tongues and assessed PNI histology. Furthermore, transcriptome profiles of the cell lines were analyzed to identify cell line\u2010specific genes associated with PNI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among OSCCs, PNI cases were more prevalent in TSCC and FOMSCC. Expression analysis detected 103 DEGs in TSCC and 401 in FOMSCC. In TSCC, 42.2% of the DEGs were non\u2010coding RNAs, whereas DEGs in FOMSCC were predominantly protein\u2010coding genes enriched in pathways related to muscle contraction and fibrosis. Xenograft analysis revealed that HO\u20101\u2010u\u20101 uniquely exhibited a nerve\u2010directed invasion phenotype. Transcriptome analysis of OSCC cell lines demonstrated that HO\u20101\u2010u\u20101 was enriched in neural\u2010 and glial\u2010related pathways, consistent with its neurotropic behavior in\u00a0vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our transcriptome analysis suggested that different pathways might drive PNI in TSCC and FOMSCC, depending on their origin. We also demonstrated that HO\u20101\u2010u\u20101 is a unique OSCC cell line with strong neurotropic properties and a valuable xenograft model for OSCC PNI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.70143","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T04:30:23Z","timestamp":1776745823000},"page":"812-821","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptome Analysis and Histological Validation Identified the\n                    <scp>HO<\/scp>\n                    \u20101\u2010\n                    <scp>u<\/scp>\n                    \u20101 Cell Line as a Model for Perineural Invasion in Oral Squamous Cell Carcinoma"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5486-2021","authenticated-orcid":false,"given":"Taisuke","family":"Hani","sequence":"first","affiliation":[{"name":"Department of Pathology The Nippon Dental University, School of Life Dentistry at Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoo","family":"Kudo","sequence":"additional","affiliation":[{"name":"Department of Pathology The Nippon Dental University, School of Life Dentistry at Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiki","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Department of Pathology The Nippon Dental University, School of Life Dentistry at Tokyo  Tokyo Japan"},{"name":"Department of Endodontics The Nippon Dental University, School of Life Dentistry at Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuuichi","family":"Soeno","sequence":"additional","affiliation":[{"name":"Department of Pathology The Nippon Dental University, School of Life Dentistry at Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2016.07.016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.40165"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13213339"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201030939\u20108"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/19160216251316219"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1231104"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16213695"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2022.215610"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12318"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.23\u201000073"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010024\u201000763\u2010x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20107\u2010294"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973\u2010029X.174630"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.13069"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20100500\u20101\u2010113"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201022\u20103496"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.4102\/sajr.v27i1.2677"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537\u2010200010000\u201000038"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.848557"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.18\u201091"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201057478\u20100"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13900"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903\u2010024\u201005113\u20109"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.518"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010023\u201002539\u2010w"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.38"}],"container-title":["Journal of Oral Pathology &amp; 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