{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T08:25:40Z","timestamp":1746692740919},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,9,5]],"date-time":"2017-09-05T00:00:00Z","timestamp":1504569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science and Technology Development","award":["175034"],"award-info":[{"award-number":["175034"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Prosthodontics"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to DS, but it seems that <jats:italic>Candida<\/jats:italic> plays the key role. The aim of the study was to evaluate the denture sonication method to: (i) increase the possibility of diagnosing patients with <jats:italic>Candida<\/jats:italic>\u2010associated DS; (ii) detect and identify the mixed <jats:italic>Candida<\/jats:italic> spp., and (iii) determine the <jats:italic>Candida<\/jats:italic> colony forming units (CFU) and its possible relationship with DS severity, based on Newton's classification.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The cross\u2010sectional study conducted at the Clinic for Dental Prosthetics, Belgrade (Serbia) from June 2013 to December 2014 enrolled edentulous patients with dentures (n = 250). Patients without DS (n = 20) were the control group (CG). The patients\u2019 data were collected, and patients with DS (study group\/SG) were selected and divided into SG <jats:italic>Candida<\/jats:italic>+ and SG <jats:italic>Candida<\/jats:italic>\u2010. Based on severity of DS, the SG patients were classified in 3 groups (Newton's classification). Four sampling methods were applied to detect patients with <jats:italic>Candida<\/jats:italic>\u2010associated DS: mucosa swab, denture swab, oral rinse, and denture sonication method. The sensitivity and specificity of denture sonication method were shown using the receiver operating characteristic (ROC) curve and the area under the curve (AUC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 97 (38.8%), out of 250 clinically examined patients, DS was diagnosed. In 82 (84.5%), out of 97 mycologically examined patients, <jats:italic>Candida<\/jats:italic>\u2010associated DS was detected when denture sonication method was applied. Additionally, using the denture sonication method we observed: (i) the largest number of <jats:italic>Candida<\/jats:italic> positive patients compared to other sampling methods (<jats:italic>p<\/jats:italic> &lt; 0.0001); (ii) the highest number of <jats:italic>Candida<\/jats:italic> CFU\/ml (10<jats:sup>5<\/jats:sup>), and (iii) the possibility to detect mixed <jats:italic>Candida<\/jats:italic> cultures. The largest number of patients with <jats:italic>Candida<\/jats:italic>\u2010associated DS showed type II (60%) DS, followed by type I (21%), and type III (19%) DS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The denture sonication method is easy, accurate, and sensitive, and increases the possibility of diagnosing patients with <jats:italic>Candida<\/jats:italic>\u2010associated DS. Additionally, yeast quantification, mixed <jats:italic>Candida<\/jats:italic> spp., and non\u2010<jats:italic>albicans Candida<\/jats:italic> were detectable when cultivation on <jats:italic>Candida<\/jats:italic> CHROMagar was performed. It was not possible using conventional methods, such as swab or oral rinse.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jopr.12610","type":"journal-article","created":{"date-parts":[[2017,9,5]],"date-time":"2017-09-05T11:04:59Z","timestamp":1504609499000},"page":"580-586","source":"Crossref","is-referenced-by-count":9,"title":["Laboratory\u2010Based Investigation of Denture Sonication Method in Patients with <i>Candida<\/i>\u2010Associated Denture Stomatitis"],"prefix":"10.1111","volume":"28","author":[{"given":"Mirjana","family":"Peri\u0107","sequence":"first","affiliation":[{"name":"Department of Prosthodontics School of Dental Medicine University of Belgrade  Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"Radunovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Dental Medicine University of Belgrade  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