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This research also aimed to investigate the antifungal activity of 3 commercial mouthwashes\/oral antiseptic formulations containing 0.12% chlorhexidine, 0.07% cetylpyridinium, or 0.075% cetylpyridinium against planktonic and sessile (biofilm mode) yeast cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Forty\u2010three <jats:italic>Candida<\/jats:italic> yeasts were isolated from 32 of 70 selected patients, and the virulence factors of <jats:italic>C. albicans<\/jats:italic>, <jats:italic>C. krusei<\/jats:italic>, <jats:italic>C. glabrata<\/jats:italic>, <jats:italic>C. tropicalis<\/jats:italic>, and <jats:italic>C. parapsilosis<\/jats:italic> species were investigated by polymerase chain reaction (PCR) and proteinase in plates. Minimum inhibitory concentration (MIC), and in vitro biofilm assay evaluated the antifungal activity of antiseptics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>C. albicans<\/jats:italic>, <jats:italic>C. krusei<\/jats:italic>, <jats:italic>C. glabrata<\/jats:italic>, <jats:italic>C. tropicalis<\/jats:italic>, and <jats:italic>C. parapsilosis<\/jats:italic> were detected in mono and mixed cultures. Only <jats:italic>C. albicans<\/jats:italic> displayed genes related to adhesion and proteinases (ALS2, ALS3, SAP1, and SAP3). The aspartate proteinase activity was found in 60.46% of isolates. The tested antiseptic formulations exhibited a MIC less than 1.25% toward yeasts in the planktonic mode. According to XTT ((2,3\u2010bis(2\u2010methoxy\u20104\u2010nitro\u20105\u2010sulfophenyl)\u20102H\u2010tetrazolium\u20105\u2010carboxanilide) assay results, most <jats:italic>Candida<\/jats:italic> isolates and all mixed cultures formed biofilms within 24 hours. The evaluated antiseptic formulations were also active against biofilms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Most virulence factors investigated here (ALS2, ALS3, SAP1, and SAP3) occurred in the majority of the <jats:italic>Candida<\/jats:italic> spp. isolates, especially in <jats:italic>C. albicans<\/jats:italic>. The tested mouthwash formulations were effective against all the yeast isolates in both the planktonic and sessile growth modes. Developing alternative therapies that can avoid or control biofilm formation is necessary to prevent oral candidiasis and other <jats:italic>Candida<\/jats:italic> spp. infections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jopr.13037","type":"journal-article","created":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T12:04:52Z","timestamp":1550232292000},"page":"398-408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Susceptibility to Oral Antiseptics and Virulence Factors Ex Vivo Associated with <i>Candida<\/i> spp. Isolated from Dental Prostheses"],"prefix":"10.1111","volume":"28","author":[{"given":"Amir Horiquini","family":"Barbosa","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences University of S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaqueline 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