{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T14:25:52Z","timestamp":1762957552455,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Prosthodontics"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>There is a need for effective solutions for the persistent prevalence of denture stomatitis (DS) in denture\u2010wearing populations. This study assessed the impact of nanohydroxyapatite (nanoHAP) nanoparticles on <jats:italic>Candida albicans<\/jats:italic> biofilm formation on milled polymethylmethacrylate (PMMA) denture bases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Acquired salivary pellicle was formed on disks milled from a prepolymerized PMMA puck. The disks were assigned to two groups, A (<jats:italic>n<\/jats:italic>\u00a0=\u00a018) and B (<jats:italic>n<\/jats:italic>\u00a0=\u00a018), and each group was further subdivided into three subgroups (6\/subgroup), each treated with either Polident solution (antibacterial denture cleanser), nanoHAP mouthwash, or distilled water. Disks in group A subgroups were soaked in their respective treatment solutions for 8\u00a0h, followed by biofilm formation on the\u00a0disk for 24\u00a0h. Disks in group B subgroups had <jats:italic>C. albicans<\/jats:italic> biofilm formed on them for 24\u00a0h, followed by soaking in their respective treatment solutions for 8\u00a0h. Biofilm thickness, biomass, and live\/dead cell ratio were determined using a\u00a0confocal laser scanning microscope. Biofilm morphology was examined with a\u00a0scanning electron microscope (SEM). Data were analyzed by ANOVA and Tukey\u2013Kramer multiple comparisons (<jats:italic>\u03b1<\/jats:italic>\u00a0=\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In group A, nanoHAP mouthwash displayed significant anti\u2010adhesive properties. In group B, biofilm biomass and thickness significantly decreased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), with the nanoHAP showing the most substantial reduction in existing biofilm compared to other solutions, but it did not significantly affect cell viability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrated the efficacy of nanoHAP mouthwash in inhibiting <jats:italic>C. albicans<\/jats:italic> biofilm formation when used as a storage medium for acrylic dentures. It suggests its potential clinical application for preventing denture stomatitis in patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jopr.13965","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T03:59:08Z","timestamp":1729223948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Influence of nanohydroxyapatite mouthwash on the growth of <i>Candida albicans<\/i> biofilm on milled denture surfaces: An in vitro study"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2535-9664","authenticated-orcid":false,"given":"Cynthia U.","family":"Udeh","sequence":"first","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5097-4466","authenticated-orcid":false,"given":"Bennett T.","family":"Amaechi","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parveez A.","family":"Abdul\u2010Azees","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amos C.","family":"Obiefuna","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Yen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"},{"name":"Department of Family Dentistry and Oral Diagnosis School of Dentistry Tri\u2010Service General Hospital and National Defense Medical Center Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nahid","family":"IftiKhar","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil","family":"Mankar","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Temitope O.","family":"Omosebi","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry Lagos State University Teaching Hospital Ikeja Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tejal","family":"Gohil","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Dentistry School of Dentistry University of Texas Health Science Center at San Antonio San Antonio Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"981","DOI":"10.18203\/2394-6040.ijcmph20220003","article-title":"Epidemiology, etiology, and treatment of denture stomatitis","volume":"9","author":"Bukhari MA","year":"2022","journal-title":"Int J Community Med Public 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