{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T04:12:54Z","timestamp":1783656774062,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Prosthodontics"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>This study aimed to characterize a mucoadhesive gel containing nystatin (NYS) or chlorhexidine (CHX), complexed or not with \u03b2\u2010cyclodextrin (\u03b2CD), and evaluate its rheological and mucoadhesive properties, antifungal efficacy, cytocompatibility, and collagen synthesis potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Mucoadhesive formulations were produced using chitosan and hydroxyethylcellulose, incorporating NYS or CHX at 32\u00a0mg\/g, or complexed with \u03b2CD (NYS:\u03b2CD, equivalent to 16.1\u00a0mg\/g of NYS, and CHX:\u03b2CD, equivalent to 4.8\u00a0mg\/g of CHX), and compared to a pure gel (GEL) and a commercially available 2% miconazole gel (DK). The formulations were evaluated for their rheological and mucoadhesive properties; antifungal activity against\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      by halo inhibition, XTT metabolic assay, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC); cytocompatibility in an organotypic oral mucosa model by LIVE\/DEAD and Alamar Blue metabolic assay; and collagen synthesis potential by fluorometric quantification. All microbiological and cytocompatibility tests were performed using mucoadhesive leachates. Statistical analysis was performed (\n                      <jats:italic>\u03b1<\/jats:italic>\n                      = 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Elastic behavior was predominant in the formulations, and greater mucoadhesiveness was observed in the complexed groups (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Limited antifungal activity was found in the GEL and DK groups, while the NYS, NYS:\u03b2CD, CHX, and CHX:\u03b2CD formulations exhibited significantly superior antifungal efficacy (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). While formulations containing CHX significantly reduced cellular metabolic activity, those containing NYS demonstrated better cytocompatibility and increased collagen production.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The mucoadhesive gel containing NYS:\u03b2CD optimized both antifungal activity without compromising its rheological, mucoadhesive properties, and cytocompatibility at lower therapeutic doses compared to noncomplexed NYS. These findings suggest significant advantages for the treatment of oral candidiasis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jopr.70135","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T09:04:57Z","timestamp":1775898297000},"page":"898-909","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization, antifungal potential, cytocompatibility, and regenerative potential of mucoadhesive gel containing antifungal\u2010\u03b2\u2010cyclodextrin inclusion complexes"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8934-4472","authenticated-orcid":false,"given":"Carolina Yoshi Campos","family":"Sugio","sequence":"first","affiliation":[{"name":"Department of Prosthodontics and Periodontics Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo Brazil"},{"name":"Department of Removable Prosthodontics and Dental Materials Dental School, Federal University of Uberl\u00e2ndia (UFU)  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Aparecida Maia Neves","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1485-6104","authenticated-orcid":false,"given":"Diana Gabriela","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Balestrero","family":"Cassiano","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Alves","family":"de Mattos","sequence":"additional","affiliation":[{"name":"Department of Dentistry State University of Ponta Grossa (UEPG)  Ponta Grossa Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics and Periodontics Bauru School of Dentistry University of S\u00e3o Paulo (USP)  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4285-6646","authenticated-orcid":false,"given":"Priscileila Colerato","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences State University of Ponta Grossa (UEPG)  Ponta Grossa Paran\u00e1 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