{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:30:02Z","timestamp":1791412202298,"version":"4.3.3"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001515","name":"Cure Kids","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Paediatrics Child Health"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aimed to describe the burden of disease and estimated rates of oropharyngeal carriage of <jats:italic>Kingella kingae<\/jats:italic> among New Zealand children. We compared polymerase chain reaction (PCR) and culture for the detection of this microorganism with a view to further development and implementation of <jats:italic>K. kingae<\/jats:italic> <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in Christchurch Hospital.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Oropharyngeal swabs from children between 6 and 48 months of age were analysed by culture to estimate carriage rates of <jats:italic>K. kingae<\/jats:italic>. Samples of a subgroup of children between 12 and 24 months of age were also tested by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. In addition, a retrospective review was performed on all cases of invasive <jats:italic>K. kingae<\/jats:italic> disease and children with osteoarticular infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Oropharyngeal cultures were positive for <jats:italic>K. kingae<\/jats:italic> in specimens from 4 out of 176 children (2.3%). <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was significantly more sensitive and by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, the carriage rate rose to 22.9% (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> = 9.4\u201333.9%) (<jats:italic>n<\/jats:italic> = 48). From 2005 to 2015, 17 children between 6 and 48 months of age were identified with invasive infections due to <jats:italic>K. kingae<\/jats:italic>. Seventy\u2010four children were found to have an osteoarticular infection. Most of these were culture\u2010negative with a microbiological diagnosis made in only 15 cases (20.3%), only one due to <jats:italic>K. kingae<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We found a very high carriage rate of <jats:italic>K. kingae<\/jats:italic> in New Zealand children and poor performance of <jats:italic>K. kingae<\/jats:italic> culture. It is likely that many cases of invasive <jats:italic>K. kingae<\/jats:italic> infections remain undetected. We recommend the use of a <jats:italic>K. kingae<\/jats:italic> <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in all children under 4 years of age with a possible osteoarticular infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jpc.13287","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T03:53:21Z","timestamp":1472788401000},"page":"1081-1085","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["High rate of oropharyngeal <i>Kingella kingae<\/i> carriage in New Zealand children"],"prefix":"10.1111","volume":"52","author":[{"given":"Laudi","family":"Olijve","sequence":"first","affiliation":[{"name":"Department of Paediatrics University of Otago  Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roslyn","family":"Podmore","sequence":"additional","affiliation":[{"name":"Microbiology Department Canterbury Health Laboratories  Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor","family":"Anderson","sequence":"additional","affiliation":[{"name":"Microbiology Department Canterbury Health Laboratories  Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Walls","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University of Otago  Christchurch New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000259954.88139.f4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00144-09"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/BPO.0b013e3181d4732f"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00028-14"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318184dbb8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4180-4184.2002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3182755779"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2015.133"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/pit003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/376913"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063181"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.070060-0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000000303"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.5.1426-1427.1995"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2005.tb01911.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.30.5.1278-1281.1992"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03799.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3182867f5e"}],"container-title":["Journal of Paediatrics and Child Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpc.13287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpc.13287","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpc.13287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:18:54Z","timestamp":1695489534000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpc.13287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":18,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/jpc.13287"],"URL":"https:\/\/doi.org\/10.1111\/jpc.13287","archive":["Portico"],"relation":{},"ISSN":["1034-4810","1440-1754"],"issn-type":[{"value":"1034-4810","type":"print"},{"value":"1440-1754","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"2016-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}