{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:38:03Z","timestamp":1747186683878,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T00:00:00Z","timestamp":1613692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Paediatrics Child Health"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>The diagnosis of coronavirus disease 2019 (COVID\u201019) depends on accurate and rapid testing. Choosing an appropriate sample may impact diagnosis. Naso\u2010oropharyngeal swabs (NOS) are most frequently used, despite several limitations. Since studies suggest nasopharyngeal aspirate (NPA) as a superior alternative in children, we hypothesised collecting both nasopharyngeal swab and aspirate would improve our diagnostic accuracy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Observational, longitudinal, prospective study from 7 March to 7 May in a tertiary paediatric hospital in Lisbon. The objective was to compare the rate of detection of SARS\u2010CoV\u20102 between NOS and NPA samples collected simultaneously.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 438 samples collected from 85 patients with confirmed COVID\u201019. There were 47.7% overall positive specimens \u2013 32% (70\/219) positive NOS and 63.5% (139\/219) positive NPA. The tests were 67.6% concordant (<jats:italic>k<\/jats:italic>\u00a0=\u20090.45). 50.3% had positive NPA with negative NOS, while 1.3% had positive NOS with negative NPA. NPA proved to be more sensitive (98.6% with 95% confidence interval 91.2\u201399.9% vs. 49.6% with 95% confidence interval 41.1\u201358.2%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u20090.001). Additionally, the difference between NPA and NOS positive samples was statistically significant across all population groups (age, health condition, clinical presentation, contact with COVID\u201019 patients or need for hospitalisation), meaning NPA is more sensitive overall.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Nasopharyngeal aspirates had greater sensitivity than naso\u2010oropharyngeal swabs in detecting SARS\u2010CoV\u20102. Our results suggest paediatric patients would benefit from collecting nasopharyngeal aspirates in hospital settings, whenever feasible, to improve diagnosis of COVID\u201019.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jpc.15405","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T00:53:14Z","timestamp":1613868794000},"page":"1078-1081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of nasopharyngeal samples for <scp>SARS\u2010CoV<\/scp>\u20102 detection in a paediatric cohort"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3545-8834","authenticated-orcid":false,"given":"Jorge","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Infectious Diseases Unit, Department of Pediatrics, Hospital Dona Estef\u00e2nia Centro Hospitalar Universit\u00e1rio Lisboa Central, EPE  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catarina","family":"Gouveia","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Department of Pediatrics, Hospital Dona Estef\u00e2nia Centro Hospitalar Universit\u00e1rio Lisboa Central, EPE  Lisbon Portugal"},{"name":"Nova Medical School Faculdade de Ci\u00eancias M\u00e9dicas  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madalena Almeida","family":"Santos","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory, Department of Clinical Pathology Centro Hospitalar Universit\u00e1rio Lisboa Central, EPE  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Costa","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory, Department of Clinical Pathology Centro Hospitalar Universit\u00e1rio Lisboa Central, EPE  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"C\u00f4rte\u2010Real","sequence":"additional","affiliation":[{"name":"Molecular Biology Laboratory, Department of Clinical Pathology Centro Hospitalar Universit\u00e1rio Lisboa Central, EPE  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Jo\u00e3o","family":"Brito","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Department of Pediatrics, Hospital Dona Estef\u00e2nia Centro Hospitalar Universit\u00e1rio Lisboa Central, EPE  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-4642(20)30177-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000690"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000002700"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2020.4573"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02593-20"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102903"},{"key":"e_1_2_6_9_1","first-page":"1843","article-title":"Detection of SARS\u2010CoV\u20102 in different types of clinical specimens","volume":"323","author":"Wang W","year":"2020","journal-title":"JAMA"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1745095"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2016359"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2020-003053"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.00465"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000002824"},{"key":"e_1_2_6_15_1","first-page":"1","article-title":"Diagnosis of SARS\u2010CoV\u20102 in children: Accuracy of nasopharyngeal swab compared to nasopharyngeal aspirate","volume":"7","author":"Di Pietro GM","year":"2021","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-0691"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01209-08"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-009-0865-7"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2020.101920"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30783-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2020.05.037"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035797"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamasurg.2020.6643"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.08.011"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2020.115130"}],"container-title":["Journal of Paediatrics and Child Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpc.15405","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpc.15405","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpc.15405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T21:53:21Z","timestamp":1692654801000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpc.15405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,19]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1111\/jpc.15405"],"URL":"https:\/\/doi.org\/10.1111\/jpc.15405","archive":["Portico"],"relation":{},"ISSN":["1034-4810","1440-1754"],"issn-type":[{"type":"print","value":"1034-4810"},{"type":"electronic","value":"1440-1754"}],"subject":[],"published":{"date-parts":[[2021,2,19]]},"assertion":[{"value":"2020-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}