{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T17:46:18Z","timestamp":1763142378098,"version":"3.32.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Paediatrics Child Health"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>Vitamin D is an essential micronutrient for multiple physiological processes, and its deficiency remains a world\u2010wide public health problem that cannot be ignored. Dried blood spot (DBS) is a convenient tool in large\u2010scale epidemiological studies, but its application in evaluating vitamin D status in Chinese population is still scarce. Herein, we aimed to determine the vitamin D status in Chinese pre\u2010school children using DBS coupled with LC\u2010MS\/MS method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We first developed a sensitive and reliable method for the determination of 25\u2010hydroxyvitamin D (25(OH)D) in DBS samples using an ultra\u2010high\u2010performance liquid chromatograph coupled with triple quadrupole mass spectrometer (UHPLC\u2010QQQ\u2010MS\/MS). Next, we conducted a pilot study to compare the 25(OH)D concentration in DBS and serum samples. Finally, the assay method was used to evaluate vitamin D status in Chinese pre\u2010school children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The present method was validated to be reliable and robust for the determination of 25(OH)D in DBS samples. Comparable consistency was observed between the 25(OH)D concentration in DBS and serum samples. A total of 3826 DBS samples collected from children aged 1\u20137\u2009years were determined. The median concentration of 25(OH)D was 19.57\u2009ng\/mL (interquartile range 14.73\u201324.36\u2009ng\/mL), and decreased from 1 to 7\u2009years of age. In addition, 13.51% of male children and 15.12% female children are found to be deficient in 25(OH)D.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>DBS coupled with LC\u2010MS\/MS is a feasible strategy to evaluate vitamin D status in epidemiological studies. And vitamin D deficiency remains a common health problem in Chinese pre\u2010school children.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jpc.16698","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T06:49:40Z","timestamp":1729147780000},"page":"20-25","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluating vitamin D status in Chinese pre\u2010school children using dried blood spots coupled with liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1111","volume":"61","author":[{"given":"Zhenxuan","family":"Zhan","sequence":"first","affiliation":[{"name":"Joint Laboratory of Shantou University Medical College and Guangdong Hybribio Biotech Ltd Shantou University Medical College  Shantou China"},{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Quan","sequence":"additional","affiliation":[{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Zhao","sequence":"additional","affiliation":[{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Mai","sequence":"additional","affiliation":[{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Joint Laboratory of Shantou University Medical College and Guangdong Hybribio Biotech Ltd Shantou University Medical College  Shantou China"},{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yudong","family":"Li","sequence":"additional","affiliation":[{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Sun","sequence":"additional","affiliation":[{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8644-0039","authenticated-orcid":false,"given":"Xuan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Joint Laboratory of Shantou University Medical College and Guangdong Hybribio Biotech Ltd Shantou University Medical College  Shantou China"},{"name":"Hybribio Medical Laboratory Group Ltd  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1024\/0300-9831\/a000733"},{"key":"e_1_2_7_3_1","first-page":"587","article-title":"Expert consensus on the standardization of liquid chromatography\u2010tandem mass spectrometry method for the measurement of 25\u2010hydroxyvitamin D","volume":"44","author":"Chinese Association of Geriatric Research of Laboratory Medicine, Chinese Geriatrics Society of Laboratory Medicine","year":"2021","journal-title":"Chin. 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Pharmacopoeia of the People's Republic of China","year":"2020","author":"Pharmacopoeia Commission of the People's Republic of China","key":"e_1_2_7_19_1"},{"volume-title":"National Health Commission of the People's Republic of China. Method for Vitamin D Deficiency Screening","year":"2020","key":"e_1_2_7_20_1"},{"key":"e_1_2_7_21_1","first-page":"359","article-title":"Clinical study on vitamin D combined with alfacalcidol in treatment of osteoporosis","volume":"36","author":"Zhao D","year":"2021","journal-title":"Drug. Clin."},{"key":"e_1_2_7_22_1","first-page":"1282","article-title":"Standardization of clinical application of mass spectrometry method for measurement of vitamin D in capillary blood of children: A multicenter study","volume":"60","author":"Li L","year":"2022","journal-title":"Chin. J. 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