{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T16:56:15Z","timestamp":1776704175351,"version":"3.51.2"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Paediatrics Child Health"],"published-print":{"date-parts":[[2025,4]]},"abstract":"ABSTRACT<\/jats:title>Aim<\/jats:title>To determine the utility of anti\u2010tissue transglutaminase IgA antibodies (tTG\u2010IgA) and anti\u2010deaminated gliadin peptide IgG antibodies (DGP\u2010IgG) in detecting coeliac disease (CD) and whether DGP\u2010IgG can replace anti\u2010endomysial IgA antibody in the European Society for Paediatric Gastroenterology Hepatology and Nutrition no\u2010biopsy approach in diagnosing CD.<\/jats:p><\/jats:sec>Methods<\/jats:title>Children aged <\u200919\u2009years who had paired tTG\u2010IgA and DGP\u2010IgG performed and had a gastroscopy with biopsies between 1 March 2016 and 31 October 2020 were retrospectively reviewed.<\/jats:p><\/jats:sec>Results<\/jats:title>Of 1206 patients who fulfilled the study criteria, 298 (24.7%) patients were diagnosed with CD. Fifteen patients with IgA deficiency were excluded from any tTG\u2010IgA analysis. tTG\u2010IgA had sensitivity and specificity of 93.5% and 92.0%, respectively, in detecting CD, while DGP\u2010IgG had sensitivity of 97.0% and specificity of 86.7%. tTG\u2010IgA \u2265\u200910x upper limit of normal (ULN) alone had a specificity of 99.3% and a positive predictive value (PPV) of 96.8%. An optimal DGP\u2010IgG threshold was identified to be 70\u2009U\/mL (3.5x ULN) based on >\u200999% specificity in detecting CD. When tTG\u2010IgA \u2265\u200910x ULN was combined with DGP\u2010IgG \u2265\u20093.5 ULN, the PPV in diagnosing CD was 98.5%. DGP\u2010IgG performed well in detecting CD in 126 children aged <\u20093\u2009years, with all patients with CD having an elevated DGP\u2010IgG (sensitivity 100%).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Combined tTG\u2010IgA \u2265\u200910x ULN and DGP\u2010IgG \u2265\u20093.5x ULN provided a high PPV (98.5%) in diagnosing CD. DGP\u2010IgG testing can potentially replace EMA testing in those children with tTG\u2010IgA \u2265\u200910x ULN. Future studies should evaluate DGP\u2010IgG testing as a sequential test.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jpc.16801","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T16:03:21Z","timestamp":1738339401000},"page":"628-634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["No\u2010Biopsy Diagnosis of Coeliac Disease in Children Without Anti\u2010Endomysial IgA<\/scp> Antibody Testing: Combining Anti\u2010Tissue Transglutaminase IgA<\/scp> and Anti\u2010Deamidated Gliadin IgG<\/scp> Antibodies"],"prefix":"10.1111","volume":"61","author":[{"given":"Marsus","family":"Pumar","sequence":"first","affiliation":[{"name":"Immunology Laboratory, Laboratory Services, Department of Allergy and Immunology The Royal Children's Hospital Melbourne Melbourne Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Choo","sequence":"additional","affiliation":[{"name":"Immunology Laboratory, Laboratory Services, Department of Allergy and Immunology The Royal Children's Hospital Melbourne Melbourne Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5787-437X","authenticated-orcid":false,"given":"Jeremy","family":"Rosenbaum","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Clinical Nutrition The Royal Children's Hospital Melbourne Melbourne Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Alex","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Clinical Nutrition The Royal Children's Hospital Melbourne Melbourne Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaun S. C.","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Clinical Nutrition The Royal Children's Hospital Melbourne Melbourne Australia"},{"name":"Murdoch Children's Research Institute Melbourne Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071600"},{"issue":"1","key":"e_1_2_12_3_1","first-page":"142","article-title":"Celiac Disease: A Comprehensive Current Review","volume":"17","author":"Caio G.","year":"2019","journal-title":"BioMed Central Medicine"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31821a23d0"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000002497"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpc.16227"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10354-024-01045-9"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-021-04068-1"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2020.05.002"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000003022"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.04.023"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2013-1099"},{"issue":"17","key":"e_1_2_12_13_1","first-page":"1662","article-title":"New Screening Tests Enrich Anti\u2010Transglutaminase Results and Support a Highly Sensitive Two\u2010Test Based Strategy for Celiac Disease Diagnosis","volume":"412","author":"Basso D.","year":"2011","journal-title":"Clinical Chemistry and Laboratory Medicine"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13317-011-0020-1"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2009.04039.x"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365521.2016.1218540"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000001460"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000002065"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.06.002"}],"container-title":["Journal of Paediatrics and Child Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpc.16801","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T04:15:13Z","timestamp":1744863313000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpc.16801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/jpc.16801"],"URL":"https:\/\/doi.org\/10.1111\/jpc.16801","archive":["Portico"],"relation":{},"ISSN":["1034-4810","1440-1754"],"issn-type":[{"value":"1034-4810","type":"print"},{"value":"1440-1754","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,31]]},"assertion":[{"value":"2023-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}