{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:53:55Z","timestamp":1791381235265,"version":"4.3.3"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Paediatrics Child Health"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The study evaluated neonatal cerebro\u2010spinal fluid (CSF) leucocyte esterase (LE) estimation by reagent strips to diagnose CSF leucocytosis in\u00a0situations where the CSF leucocyte count is delayed or unavailable.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This prospective diagnostic study had a cohort\u2010selection design. CSF samples of neonates with clinical sepsis were analysed for LE by reagent strips, cytochemistry, gram stain and culture. CSF LE was measured on an ordinal scale from nil to large. Correlations of CSF LE with CSF leucocyte and neutrophil count were determined. CSF LE was compared versus CSF leucocytosis (defined a priori \u2265\u200925 leucocytes\/\u03bcL) and versus CSF neutrophilia (defined a priori \u2265\u200915 neutrophils\/\u03bcL) as the reference standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 341 neonates were enrolled. CSF LE values correlated with leucocyte count (rho\u2009=\u20090.49,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and neutrophil count (rho\u2009=\u20090.44,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). LE had a sensitivity (95% CI) of 63.5% (51.2, 74.1), specificity 90.7% (88.4, 92.6), LR+ (likelihood ratio) 6.79 (4.43, 9.98) and LR\u2212 0.40 (0.28, 0.55) to diagnose CSF leucocytosis. When expressed as an ordinal variable, LE had an area under the receiver operator characteristic curve of 0.77 (95% CI: 0.69, 0.85;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) to detect leucocytosis. With respect to all parameters, the diagnostic performance of CSF LE to detect neutrophilia was inferior to the detection of leucocytosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      CSF leucocyte esterase estimation by reagent strips has a moderately high specificity and a good LR\n                      <jats:sup>+<\/jats:sup>\n                      but, overall, does not have sufficiently robust diagnostic accuracy to substitute CSF leucocyte counts.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jpc.70217","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:37:48Z","timestamp":1761219468000},"page":"1894-1900","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leukocyte Esterase Estimation to Diagnose Cerebro\u2010Spinal Fluid Leucocytosis in Neonates"],"prefix":"10.1111","volume":"61","author":[{"given":"Sourabh","family":"Dutta","sequence":"first","affiliation":[{"name":"Department of Pediatrics Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnab","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry PGIMER  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallab","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology PGIMER  Chandigarh 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