{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:18:05Z","timestamp":1792801085523,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Paediatrics Child Health"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Meningoencephalitis causes significant morbidity and mortality in children worldwide. Prompt diagnosis remains challenging, yet advances in molecular diagnostic technology have improved diagnostic performance. This study examined whether the introduction of the BioFire FilmArray meningitis\/encephalitis (ME) multiplex panel impacted the diagnosis and management of children with suspected meningoencephalitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a retrospective cohort study (January 2019 to July 2021) at a non\u2010tertiary hospital in Sydney, Australia, during which the BioFire ME panel became available. Patients\u2009&lt;\u200916\u2009years who had cerebrospinal fluid (CSF) sampling for investigation of meningoencephalitis were included. Demographic, clinical and microbiological data were extracted to evaluate time to infection diagnosis, antimicrobial rationalisation and duration, investigations undertaken and admission length.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There were 122 CSF samples collected from children with suspected meningoencephalitis; 70\/122 (57.3%) before and 52\/122 (42.7%) after BioFire ME panel introduction. Twenty\u2010eight (23.0%) children had BioFire ME testing. Seven bacterial and 27 viral microbiologically confirmed central nervous system (CNS) infections were identified. BioFire ME panel use was associated with faster median confirmation or exclusion of meningoencephalitis (12.6 vs. 48.0\u2009h,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01, for bacterial and 12.6 vs. 71.5\u2009h,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01, for viral causes), quicker pathogen identification in viral meningoencephalitis (8.1 vs. 48.7\u2009h,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.019) and earlier antimicrobial rationalisation (24.0 vs. 30.4\u2009h,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Total antimicrobial duration, investigation numbers and hospitalisation duration were not reduced.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The BioFire ME panel has the potential to reduce the duration of empiric broad\u2010spectrum antimicrobials and their associated adverse effects on children, as well as improve healthcare resource efficiencies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jpc.70303","type":"journal-article","created":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:11:15Z","timestamp":1770444675000},"page":"586-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of the\n                    <scp>BioFire FilmArray<\/scp>\n                    Meningitis\u2010Encephalitis Panel on Management of Suspected Paediatric Central Nervous System Infections: A Single\u2010Centre Retrospective Cohort Study"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6330-4238","authenticated-orcid":false,"given":"Louisa Mary","family":"Churcher","sequence":"first","affiliation":[{"name":"Department of Paediatrics Northern Beaches Hospital  Sydney New South Wales Australia"},{"name":"School of Women and Children's Health University of New South Wales  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7532-9316","authenticated-orcid":false,"given":"Adam William","family":"Bartlett","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Sydney Children's Hospital  Randwick New South Wales Australia"},{"name":"The Kirby Institute University of New South Wales  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0288-8644","authenticated-orcid":false,"given":"Stella","family":"Pendle","sequence":"additional","affiliation":[{"name":"Department of Microbiology Australian Clinical Labs  Bella Vista New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9995-8846","authenticated-orcid":false,"given":"Rob","family":"Slade","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Northern Beaches Hospital  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2250-0594","authenticated-orcid":false,"given":"Phoebe C. M.","family":"Williams","sequence":"additional","affiliation":[{"name":"School of Women and Children's Health University of New South Wales  Sydney New South Wales Australia"},{"name":"Department of Infectious Diseases Sydney Children's Hospital  Randwick New South Wales Australia"},{"name":"Sydney Institute for Infectious Diseases, School of Public Health, Faculty of Medicine University of Sydney  Sydney New South Wales Australia"},{"name":"National Centre for Immunisation Research and Surveillance  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70306-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9020377"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz325"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aad0917"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30297-X"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/emj.2009.075598"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2018.07.004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00024-17"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.11.016"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01927-17"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2022.101275"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.05.017"},{"key":"e_1_2_11_14_1","unstructured":"BioM\u00e9rieux \u201cBIOFIRE FILMARRAY Meningitis\/Encephalitis (ME) Panel \u201d2024 December 5 https:\/\/www.biomerieux.com\/us\/en\/our\u2010offer\/clinical\u2010products\/biofire\u2010meningitis\u2010encephalitis\u2010panel.html."},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11081028"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2019.114935"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000003812"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12887-020-1944-2"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-03986-6"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000001936"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu232"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-002-0867-5"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2015.01.023"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41429-023-00687-y"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365521.2017.1291719"}],"container-title":["Journal of Paediatrics and Child Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpc.70303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpc.70303","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpc.70303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T13:56:13Z","timestamp":1775138173000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpc.70303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,6]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/jpc.70303"],"URL":"https:\/\/doi.org\/10.1111\/jpc.70303","archive":["Portico"],"relation":{},"ISSN":["1034-4810","1440-1754"],"issn-type":[{"value":"1034-4810","type":"print"},{"value":"1440-1754","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,6]]},"assertion":[{"value":"2025-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}