{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:09:31Z","timestamp":1768781371263,"version":"3.49.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7122176"],"award-info":[{"award-number":["7122176"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry","award":["20101561"],"award-info":[{"award-number":["20101561"]}]},{"name":"Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry","award":["NCET-11-0114"],"award-info":[{"award-number":["NCET-11-0114"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The main purpose of this study was to enhance the intestinal absorption activity and hepatoprotective effect of herpetrione by drug nanosuspensions.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Herpetrione nanosuspensions (HNS) were prepared using pH-dependent dissolving-precipitating\/homogenization process and then systematically characterized. The intestinal absorption activity of HNS were studied using the recirculating perfusion technique in comparison with herpetrione coarse suspensions (HCS) and pure herpetrione using the recirculating perfusion technique. The protective effect of HNS against acute liver injury induced by carbon tetrachloride (CCl4) in mice was also investigated and compared with that of HCS.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>The mean particle size of HNS was 269 \u00b1 7 nm with a polydispersity index of 0.187 \u00b1 0.021. The result of X-ray powder diffraction indicated that herpetrione was in amorphous state in both coarse powder and nanosuspensions. The intestinal absorption activity of HNS were superior to the HCS and pure herpetrione. As evidenced by the lowering of serum aminotransferase levels and the improvement of the degree of liver lesion, pretreatment with HNS markedly enhanced the hepatoprotective effect of herpetrione against acute liver injury induced by CCl4 in mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>HNS prepared using pH-dependent dissolving-precipitating\/homogenization technique are able to significantly enhance the intestinal absorption activity and the hepatoprotective effect of herpetrione due to the particle size reduction.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.12103","type":"journal-article","created":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T02:02:40Z","timestamp":1373853760000},"page":"1382-1392","source":"Crossref","is-referenced-by-count":6,"title":["Enhanced intestinal absorption activity and hepatoprotective effect of herpetrione via preparation of nanosuspensions using pH-dependent dissolving-precipitating\/homogenization process"],"prefix":"10.1093","volume":"65","author":[{"given":"Baode","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]},{"name":"Key Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine , Nanchang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiying","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyuan","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shixiao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]},{"name":"Key Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine , Nanchang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]},{"name":"Key Lab of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine , Nanchang,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hailong","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, 302 Military Hospital , Beijing,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,9,1]]},"reference":[{"key":"2024103109174285400_jphp12103-cit-0001","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ejpb.2005.05.009","article-title":"Drug nanocrystals of poorly soluble drugs produced by high pressure homogenisation","volume":"62","author":"Keck","year":"2006","journal-title":"Eur J Pharm Biopharm"},{"key":"2024103109174285400_jphp12103-cit-0002","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ejpb.2008.06.014","article-title":"Adsorption of pharmaceutical excipients onto microcrystals of siramesine hydrochloride: effects on physicochemical properties","volume":"71","author":"Zimmermann","year":"2009","journal-title":"Eur J Pharm Biopharm"},{"key":"2024103109174285400_jphp12103-cit-0003","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.addr.2007.05.003","article-title":"Nanosizing-oral formulation development and biopharmaceutical evaluation","volume":"59","author":"Kesisoglou","year":"2007","journal-title":"Adv Drug Deliv Rev"},{"key":"2024103109174285400_jphp12103-cit-0004","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1021\/mp900254y","article-title":"Homogeneous nanoparticles to enhance the efficiency of a hydrophobic drug, antihyperlipidemic probucol, characterized by solid-state NMR","volume":"7","author":"Io","year":"2010","journal-title":"Mol Pharm"},{"key":"2024103109174285400_jphp12103-cit-0005","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.ejpb.2011.07.005","article-title":"Nanocrystals as tool to improve piroxicam dissolution rate in novel orally disintegrating tablets","volume":"79","author":"Lai","year":"2011","journal-title":"Eur J Pharm Biopharm"},{"key":"2024103109174285400_jphp12103-cit-0006","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ijpharm.2011.01.059","article-title":"er al. Preparation and in vitro\/in vivo evaluation of revaprazan hydrochloride nanosuspension","volume":"408","author":"Li","year":"2011","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0007","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/nrd1494","article-title":"Nanosuspensions in drug delivery","volume":"3","author":"Rabinow","year":"2004","journal-title":"Nat Rev Drug Discov"},{"key":"2024103109174285400_jphp12103-cit-0008","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0928-0987(02)00251-8","article-title":"Nanosizing: a formulation approach for poorly-water-soluble compounds","volume":"18","author":"Merisko-Liversidge","year":"2003","journal-title":"Eur J Pharm Sci"},{"key":"2024103109174285400_jphp12103-cit-0009","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.cocis.2004.06.009","article-title":"Current strategies for engineering drug nanoparticles","volume":"9","author":"Date","year":"2004","journal-title":"Curr Opin Colloid Interface Sci"},{"key":"2024103109174285400_jphp12103-cit-0010","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.ijpharm.2007.04.025","article-title":"Preparation of uniform prednisolone microcrystals by a controlled microprecipitation method","volume":"342","author":"Li","year":"2007","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0011","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1615\/CritRevTherDrugCarrierSyst.v28.i5.20","article-title":"Nanosuspensions in drug delivery: recent advances, patent scenarios, and commercialization aspects","volume":"28","author":"Chavhan","year":"2011","journal-title":"Crit Rev Ther Drug Carrier Syst"},{"key":"2024103109174285400_jphp12103-cit-0012","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.ijpharm.2008.07.023","article-title":"Top-down production of drug nanocrystals: nanosuspensionstabilization, miniaturization and transformation into solid products","volume":"364","author":"Van Eerdenbrugh","year":"2008","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0013","first-page":"149","article-title":"Nanosized technological approaches for the delivery of poorly water soluble drugs","volume":"6","author":"Nanjwadea","year":"2010","journal-title":"Iranian Journal of Pharmaceutical Sciences"},{"key":"2024103109174285400_jphp12103-cit-0014","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.ijpharm.2011.09.003","article-title":"Process optimization of a novel production method for nanosuspensions using design of experiments (DoE)","volume":"420","author":"Salazar","year":"2011","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0015","article-title":"Microprecipitation method for prepareing submicron suspension","volume-title":"U.S. patent US2003\/0003155A1","author":"Kipp","year":"2003"},{"key":"2024103109174285400_jphp12103-cit-0016","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1007\/s11051-008-9357-4","article-title":"Drug nanocrystals for the formulation of poorly soluble drugs and its application as a potential drug delivery system","volume":"10","author":"Gao","year":"2008","journal-title":"J Nanopart Res"},{"key":"2024103109174285400_jphp12103-cit-0017","doi-asserted-by":"crossref","first-page":"375104","DOI":"10.1088\/0957-4484\/19\/37\/375104","article-title":"A facile nanoaggregation strategy for oral delivery of hydrophobic drugs by utilizing acid-base neutralization reactions","volume":"19","author":"Chen","year":"2008","journal-title":"Nanotechnology."},{"key":"2024103109174285400_jphp12103-cit-0018","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.ijpharm.2010.11.009","article-title":"Preparation of a chemically stable quercetin formulation using nanosuspension technology","volume":"404","author":"Gao","year":"2011","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0019","doi-asserted-by":"crossref","first-page":"5881","DOI":"10.1016\/S0040-4039(00)94226-6","article-title":"Herpetrione, a trimeric lignoid isolated from Herpetospermum caudigerum Wall","volume":"24","author":"Kaouadji","year":"1983","journal-title":"Tetrahedron Lett"},{"key":"2024103109174285400_jphp12103-cit-0020","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1248\/cpb.54.1592","article-title":"Hepatitis B virus inhibiting constituents from Herpetospermum caudigerum","volume":"54","author":"Yuan","year":"2006","journal-title":"Chem Pharm Bull"},{"key":"2024103109174285400_jphp12103-cit-0021","first-page":"1080","article-title":"Study on the chemical constitutents of Herpetospermum caudigerum","volume":"35","author":"Xu","year":"2012","journal-title":"Chin Med Mat"},{"key":"2024103109174285400_jphp12103-cit-0022","first-page":"133","volume-title":"Drug Standard of Ministry of Public Healthy of People's Republic of China, Tibetan Medicine","author":"Chinese Pharmacopoeia Committee","year":"1995"},{"key":"2024103109174285400_jphp12103-cit-0023","article-title":"Preparation and application of Herperione and its capsule","volume-title":"China patent CN102040101A","author":"Yuan","year":"2011"},{"key":"2024103109174285400_jphp12103-cit-0024","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1002\/jemt.10359","article-title":"In vitro and in situ experimental model for X-ray microanalysis of intestinal epithelium","volume":"62","author":"Vanthanouvong","year":"2003","journal-title":"Microsc Res Tech"},{"key":"2024103109174285400_jphp12103-cit-0025","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1002\/jps.20593","article-title":"Investigation of the absorption mechanisms of baicalin and baicalein in rats","volume":"95","author":"Liu","year":"2006","journal-title":"J Pharm Sci"},{"key":"2024103109174285400_jphp12103-cit-0026","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1248\/bpb.33.983","article-title":"Hepatoprotective effect of syringic acid and vanillic acid on CCl4-induced liver injury","volume":"33","author":"Itoh","year":"2010","journal-title":"Biol Pharm Bull"},{"key":"2024103109174285400_jphp12103-cit-0027","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.colsurfa.2008.07.016","article-title":"Crystallized miniemulsions: influence of operating parameters during high-pressure homogenization on size and shape of particles","volume":"331","author":"Gramdorf","year":"2008","journal-title":"Colloids Surf A Physicochem Eng Asp"},{"key":"2024103109174285400_jphp12103-cit-0028","doi-asserted-by":"crossref","first-page":"417","DOI":"10.2174\/157341309789378177","article-title":"Formulation of nanosuspensions as a new approach for the delivery of poorly soluble drugs","volume":"5","author":"Pu","year":"2009","journal-title":"Curr Nanosci"},{"key":"2024103109174285400_jphp12103-cit-0029","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1023\/A:1014276917363","article-title":"Production and characterization of a budesonide nanosuspension for pulmonary administration","volume":"19","author":"M\u00fcller","year":"2002","journal-title":"Pharm Res"},{"key":"2024103109174285400_jphp12103-cit-0030","doi-asserted-by":"crossref","first-page":"4869","DOI":"10.1002\/jps.21380","article-title":"Fluticasone and budesonide nanosuspensions for pulmonary delivery: preparation, characterization, and pharmacokinetic studies","volume":"97","author":"Yang","year":"2008","journal-title":"J Pharm Sci"},{"key":"2024103109174285400_jphp12103-cit-0031","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0378-5173(02)00040-6","article-title":"Buparvaquone mucoadhesive nanosuspension: preparation, optimisation and long-term stability","volume":"237","author":"Muller","year":"2002","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0032","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1615\/CritRevTherDrugCarrierSyst.v28.i5.20","article-title":"Nanosuspensions in drug delivery: recent advances, patent scenarios, and commercialization aspects","volume":"28","author":"Chavhan","year":"2011","journal-title":"Critical Reviews\u2122 in Therapeutic Drug Carrier Systems"},{"key":"2024103109174285400_jphp12103-cit-0033","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ijpharm.2009.01.024","article-title":"Diclofenac nanosuspensions: influence of preparation procedure and crystal form on drug dissolution behaviour","volume":"373","author":"Lai","year":"2009","journal-title":"Int J Pharm"},{"key":"2024103109174285400_jphp12103-cit-0034","doi-asserted-by":"crossref","first-page":"78","DOI":"10.2174\/157488508784221253","article-title":"Micro\/nanoparticle design and fabrication for pharmaceutical drug preparation and delivery applications","volume":"3","author":"Sahoo","year":"2008","journal-title":"Current Drug Therapy"},{"key":"2024103109174285400_jphp12103-cit-0035","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0169-409X(00)00120-4","article-title":"Mucoadhesive nanoparticulate systems for peptide drug delivery","volume":"47","author":"Takeuchi","year":"2001","journal-title":"Adv Drug Deliv Rev"},{"key":"2024103109174285400_jphp12103-cit-0036","first-page":"219","article-title":"Effect of Wuweibaoganwan on rat models of acute hepatic damage","volume":"26","author":"Zhang","year":"2011","journal-title":"Med J Qilu"},{"key":"2024103109174285400_jphp12103-cit-0037","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.jep.2009.01.027","article-title":"The protective effect of Zizyphus jujube fruit on carbon tetrachloride-induced hepatic injury in mice by anti-oxidative activities","volume":"122","author":"Shen","year":"2009","journal-title":"J Ethnopharmacol"},{"key":"2024103109174285400_jphp12103-cit-0038","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1002\/jsfa.3255","article-title":"Hepatoprotective effects of sea buckthorn (Hippophae rhamnoides L.) against carbon tetrachloride induced liver injury in rats","volume":"88","author":"Geetha","year":"2008","journal-title":"J Sci Food Agri"},{"key":"2024103109174285400_jphp12103-cit-0039","first-page":"147","article-title":"Anti-oxidant activities of the seed extracts of Herpetospermum pedunculosum against liver injury in rats","volume":"23","author":"Fang","year":"2008","journal-title":"West China Journal of Pharmaceutical Sciences"},{"key":"2024103109174285400_jphp12103-cit-0040","first-page":"196","article-title":"Progress of the preparation and applications in pharmaceutics of nanosuspensions","volume":"37","author":"Zhu","year":"2006","journal-title":"Chinese Journal of Pharmaceuticals"}],"container-title":["Journal of Pharmacy and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjphp.12103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjphp.12103","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/65\/9\/1382\/60253409\/jphp12103.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/65\/9\/1382\/60253409\/jphp12103.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T09:28:50Z","timestamp":1730366930000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jpp\/article\/65\/9\/1382\/6132938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,1]]},"references-count":40,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2013,9,1]]},"published-print":{"date-parts":[[2013,9,1]]}},"URL":"https:\/\/doi.org\/10.1111\/jphp.12103","relation":{},"ISSN":["2042-7158","0022-3573"],"issn-type":[{"value":"2042-7158","type":"electronic"},{"value":"0022-3573","type":"print"}],"subject":[],"published-other":{"date-parts":[[2013,9]]},"published":{"date-parts":[[2013,9,1]]}}}