{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:46:03Z","timestamp":1773801963551,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Research Foundation of the Education Bureau of Heibei Province","award":["QN20131046"],"award-info":[{"award-number":["QN20131046"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31401003"],"award-info":[{"award-number":["31401003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Youth Foundation of Hebei University of Chinese Medicine","award":["QNZ2015003"],"award-info":[{"award-number":["QNZ2015003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>This study investigated the effect of tannic acid (TA), a plant-derived hydrolyzable polyphenol, on Kv7.4 and Kv7.5 K+ channels and rat mesenteric artery.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Whole-cell patch clamp experiments were used to record the Kv7.4 and Kv7.3\/7.5 K+ currents expressed in HEK293 cells; and the tension changes of mesenteric arteries isolated from rats were recorded using small vessel myography apparatus.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Key findings<\/jats:title>\n                  <jats:p>Tannic acid increases the Kv7.4 and Kv7.3\/7.5 K+ currents in a concentration-dependent manner (median effective concentration (EC50) = 27.3 \u00b1 3.6 \u03bcm and EC50 = 23.1 \u00b1 3.9 \u03bcm, respectively). In addition, 30 \u03bcm TA shifts the G\u2013V curve of Kv7.4 and Kv7.3\/7.5 K+ currents to the left by 14.18 and 25.24 mV, respectively, and prolongs the deactivation time constants by 184.44 and 154.77 ms, respectively. Moreover, TA relaxes the vascular tension of rat mesenteric arteries in a concentration-dependent manner (half inhibitory concentration (IC50) = 148.7 \u00b1 13.4 \u03bcm).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>These results confirms the vasodilatory effects of TA on rat mesenteric artery and the activating effects on the Kv7.4 and Kv7.3\/7.5 K+ channels, which may be a mechanism to explain the vasodilatory effect and this mechanism can be used in the research of antihypertension.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1111\/jphp.12527","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T07:02:47Z","timestamp":1457766167000},"page":"494-502","source":"Crossref","is-referenced-by-count":14,"title":["Tannic acid activates the Kv7.4 and Kv7.3\/7.5 K+ channels expressed in HEK293 cells and reduces tension in the rat mesenteric arteries"],"prefix":"10.1093","volume":"68","author":[{"given":"Yuanyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Hebei University of Chinese Medicine , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Fourth Hospital of Hebei Medical University , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Maternal and Child Health Hospital , Tangshan, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Vascular Surgery, The East Branch of Second Hospital of Hebei Medical University , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebei University of Chinese Medicine , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebei University of Chinese Medicine , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebei University of Chinese Medicine , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongsheng","family":"Dong","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, Air Force General Hospital , Beijing, Jiangsu,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Bao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Metabolism & Pharmacokinetics, China Pharmaceutical University , Nanjing, Jiangsu,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebei University of Chinese Medicine , Shijiazhuang, Hebei,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Hebei University of Chinese Medicine , Shijiazhuang, 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